Alternative additives

1. Eugenio Dr.Vincenzi Technical Manager Sintofarm Alternative additives

2. Eugenio Dr.Vincenzi Sintofarm spa SintoMANN • SintoMANN is a prebiotic rich in mannanoligosaccharides (MOS), produced from the cell wall of a specially selected strain of Saccharomyces cerevisiae. • Highly efficient in the agglutination of pathogens, reducing risk of pathogen colonization of gut. • Modulates gut flora, with reduction of gut disorder episodes and negative impact on animals.

3. SintoMANN Eugenio Dr.Vincenzi Sintofarm spa

4. Eugenio Dr.Vincenzi Sintofarm spa SintoMANN Competition Products – A Few Results Innactive Dry Yeast – Solubles due to wall rupture caused by fast water evaporation Partial ‘Lysis’ – Low protein suggests separation of extracts to some extent, but still too much solubles Hydrolysis – High solubles, added minerals (clay), loss of mannans

5. Eugenio Dr.Vincenzi Sintofarm spa SintoMANN Average (log 10) of counts of SALMO in cecal content of broilers (3 and 6 days after infection) Furlan e Berchieri Jr., 2008

6. SintoMANN Eugenio Dr.Vincenzi Sintofarm spa

7. SintoMANN Layered CellWall Structure Yeast CellWall E.coli Eugenio Dr.Vincenzi Sintofarm spa E.coli piliattaches to SintoMANN Yeast Cell Wall E.coli pili

8. SintoMANN Eugenio Dr.Vincenzi Sintofarm spa SintoMANN agglutinates isolated strains of Vibrio ordalii and atypical Aeromonas salmonicida ¹ Bacteria were cultivated in Luria agar and TSA agar (Tripticase Soy agar). ² After incubation, 2 bacterial suspensions were produced: 0.25 (7.5X107) and 0.50 McFarland scale (1.5X108 bacteria/ml). Negative(-): absence of agglutination reaction. Mild reaction (+): focal agglutination reaction Moderate reaction (++): diffuse agglutination reaction. Don´t produce type 1 fimbriae

9. SintoMANN Eugenio Dr.Vincenzi Sintofarm spa SintoMANN works in the pathogen agglutination. Experimental Trial in vitro (Santurio, J. M.; Alves, S. M. 2005. Universidade Federal de Santa Maria). SintoMANN agglutinated pathogens GRAM -

10. SintoMANN Eugenio Dr.Vincenzi Sintofarm spa SintoMANN acts on the reduction of Clostridium perfringens. 8% Lower Mortality Positive Synergy with Antibiotic GRAM+ Experimental trial carried out with broilers challenged with Clostridium perfringens, added SintoMANN (1,5 kg/ton). (Mathis, G.F. et al. 2007. Southern Poultry Research, Inc. Athens, GA).

11. SintoMANN Eugenio Dr.Vincenzi Sintofarm spa LSD Test (P<0,05) 14% Better Feed Conversion

12. SintoMANN Eugenio Dr.Vincenzi Sintofarm spa LSD Test (P<0,05) 18% Higher Weight Gain

13. SintoMANN Eugenio Dr.Vincenzi Sintofarm spa SintoMANN acts promotes intestinal health SintoMANN increased the number of Bifidobacteria. Experimental trial carried out with broilers, with dietary inclusion of SintoMANN in the starter and grower diets(1.5 and 1.0 kg/ton, respectively). (Bushansingh, B.; Zhao, X. 2007. McGill University, Montreal, Canada).

14. SintoMANN Eugenio Dr.Vincenzi Sintofarm spa 1 SEM (Standard Error of the Mean): Mean ± SEM of 9 replicates). Test of Scheffe (P>0.05) SintoMANN improved the integrity of the intestinal mucosa.

15. SintoMANN Eugenio Dr.Vincenzi Sintofarm spa SintoMANN allows the withdrawal of antibiotic growth promoter Tukey Test (P<0,05) Experimental trial carried out with broilers, with dietary inlcusion of SintoMANN in pre-starter / starter, grower and finisher diets (1.5, 1.0 and 0.5 kg/ton, respectively)..

16. SintoMANN Eugenio Dr.Vincenzi Sintofarm spa Tukey Test (P<0,05) SintoMANN was better than competitors in jejunum villi height and weight gain.

17. SintoMANN Eugenio Dr.Vincenzi Sintofarm spa SintoMANN improves performance Duncan Test (P<0,05) Experimental trial carried out with broilers, with dietary inclusion of SintoMANN in pre starter/starter, grower, and finisher diets, (1.5; 1.0 and 0.5 kg/ton, respectively).

18. SintoMANN Eugenio Dr.Vincenzi Sintofarm spa Duncan Test (P<0,05) • SintoMANN promoted: • Weight Gain • Better Feed Conversion

19. SintoMANN Eugenio Dr.Vincenzi Sintofarm spa Assessment of SintoMANN additioned to broiler diets Improves on average the production by 3.3%.

20. SintoMANN Eugenio Dr.Vincenzi Sintofarm spa SintoMANN promotes weight gain in piglets • 18% Higher Weight Gain • 14% Better Feed Conversion • 10% Higher Body Weight

21. SintoMANN Eugenio Dr.Vincenzi Sintofarm spa SintoMANN controls the incidence of diarrhea of newly – weaned piglets Fecal score of piglets fed different levels of a prebiotic, source of mannanoligosaccharides (SintoMANN) - 23,3% of diarrhea with 1kg/MT of SintoMANN - 47,5% of diarrhea with 2kg/MT of SintoMANN

22. SintoMANN Eugenio Dr.Vincenzi Sintofarm spa SintoMANN increases the production of acetic acid and propionic in dogs Short-chain fatty acids, derived from the metabolism / differential fermentation of certain sugars, are favorable for increasing the production of energy available for the maintenance of intestinal integrity. SintoMANN increased the concentration of butyric acid, linear and showed superior effect on the production of acetic acid and propionic.

23. SintoMANN Eugenio Dr.Vincenzi Sintofarm spa SintoMANN promotes reduction of tyramine and histamine in feces of dogs SintoMANN promoted linear reduction in the concentrations of tyramine and histamine in feces and resulted in responses in the fecal production of phenyl ethylamine.

24. SintoMANN Eugenio Dr.Vincenzi Sintofarm spa SintoMANN promotes adherence of E.coli

25. SintoMANN Eugenio Dr.Vincenzi Sintofarm spa SintoMANN promotes adherence of E.coli SintoMANN is higher in agglutination of bacteria E. coli.