Lecture 16 chapter 9 promoters
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Lecture 16 Chapter 9 Promoters. Neal Stewart. Objectives. What heterologous promoters are used to make transgenic plants, and why? Why are constitutive promoters so popular? What additional vector considerations should be considered given the nature of strong constitutive promoters?.

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Lecture 16 chapter 9 promoters l.jpg

Lecture 16 Chapter 9 Promoters

Neal Stewart


Objectives l.jpg
Objectives

  • What heterologous promoters are used to make transgenic plants, and why?

  • Why are constitutive promoters so popular?

  • What additional vector considerations should be considered given the nature of strong constitutive promoters?




Types of cassettes l.jpg
Types of cassettes

Figure 9.1


Promoter and enhancer trapping l.jpg

Figure 9.5

Promoter and enhancer trapping


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Figure 9.6

Strong heterologous promoters might have “bleed-over” effects



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What would be the properties of an ideal promoter?

  • Broad range of expression in a number of diverse species

  • Of plant origin

  • Maybe constitutive

  • High expression

  • Tissue specificity

  • Developmental specificity

  • Inducibility

  • Compact—short sequence


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