بسم الله الرحمن الرحيم. Genital Culture. 2013-2014. Diagnostic Medical Microbiology-Laboratory Manual. Genital Culture. Genital culture
Who is authorized to order the test
Time relapse before processing the sample
According to the type of swab ( recommended within 30 min).
Maintain specimen swab at room temperature. Do not refrigerate.
Quantity of specimen
Sufficient amount on swab or aspiration.
KOH slide A sample of the vaginal discharge is placed on a slide and mixed with a solution of 10% potassium hydroxide (KOH). The KOH kills bacteria and cells from the vagina, leaving only yeast for easier detection of a yeast infection.
Digest of casein.
Digest of animal tissue.
Dextrose. 4 %
Agar 1.5 %
Final pH 5.6 ± 0.2 at25°C
Sabouraud Dextrose agar is used for the isolation, cultivation, and maintenance of saprophytic and pathogenic fungi.
It supplies peptone as the protein source and dextrose as the carbohydrate source for nourishment .
Bacterial suppression occur due to the low pH (5.6) pH.
Note: chloramphenicol may use to inhibit growth of bacteria.
Typical colonial morphology on Thayer-Martin Selective Agar of Neisseria gonorrhoeae .... Small, grayish-white to colorless, mucoid
In the JEMBEC system, a tablet consisting of a mixture of citric acid and sodium bicarbonate is placed in a well within the plate and is activated by the moisture (humidity) produced by the culture medium within the sealed plastic bag. The CO2 levels generated are sufficient for the growth of Neisseria gonorrhoeae on the selective medium provided with the system
Direct Immuno fluorescence (DIF) Antibody labeling for Neisseria gonorrhoeae
Direct Immuno fluorescence (DIF) Antibody labeling for Chlamydia trachomatis
With Giema stain
With Iodine stain
Giemsa stain of Chlamydia inclusion bodies (purple "caps" on epithelial cell).
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