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Host-dependent and host-independent membrane proteins in Bdellovibrio: iTRAQ analysis

This study analyzes the role of host-dependent and host-independent membrane proteins in Bdellovibrio using iTRAQ analysis. Results show differential regulation of membrane proteins in Bdellovibrio strains grown in different hosts.

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Host-dependent and host-independent membrane proteins in Bdellovibrio: iTRAQ analysis

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  1. 176.5 113.7 Bd0160 80.9 63.8 Bd3348 49.5 Bd2994 37.4 Bd1015 Bd0427 Bd0459 Bd0427 Bd0286 26.0 Bd1475 Bd1290 19.6 Bd3037 Bd0633 Bd1337 14.9 Bd2969 (L16P) Bd3340 Bd3340 Bd0010 8.4 Host-dependent membrane proteins Host-independent membrane proteins Fig 1

  2. Fig 2

  3. Average Ratio (Protein iTRAQ value in HD100 strain/ HI100 strain) Fig 3

  4. Average Ratio (Protein iTRAQ value in HD100 strain/ HI100 strain) Fig 4

  5. Average Ratio (Protein iTRAQ value in HD100 strain / HI100 strain) Fig 5

  6. Average Ratio (Protein iTRAQ value in HD100 strain / HI100 strain) Fig 6

  7. Average Ratio (Protein iTRAQ value in HD100 strain / HI100 strain) Signal transduction Energy production Membrane biogenesis Intracellular transport and secretion Post-translational modification Fig 6

  8. Intraperiplasmic stage M Ec HD HDDNA 15’ 1h 2h 3h 4h HI Bd0112 Bd0427 M: Marker Ec: E. coli S17-1 RNA only. HD : HD100 RNA only HI : HID2 RNA only HDDNA : HD100 genomic DNA Bd1337 Bd3180 Bd2611 Bd3000 Fig 7 RT-PCR analysis of candidate membrane proteins ( (Sockett Lab, UK)

  9. A Host Dependent Bdellovibrio grown on Escherichia coli (HD100-E) C Bdellovibrio Grown on Salmonella typhimurium (HD100-S) D Host Independent mutant Bdellovibrio grown on host free medium. (HID2) B Bdellovibrio Grown on Pseudomonas putida (HD100-P) HD100-E HD100-P HD100-S HID2

  10. Expt1 Expt2 Expt1 Expt1 Expt2 34 52 58 2 3 8 4 9 9 7 Total proteins identified Membrane proteins upregulated in HD strain Membrane proteins upregulated in HI strain Summary of iTRAQ analysis of Bdellovibrio membrane proteins * These proteins were upregulated in HD cells grown in atleast one host. Expt2

  11. Summary of candidate membrane proteins A : Identified in one dataset ; B: Shows similar trend in 2DGE analysis ; C : Shows similar trend in SDS-PAGE analysis;D : Shows similar trend in total protein iTRAQ analysis E: Not detected in iTRAQ analysis

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