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Development of Bleaching Reagent Testing by Using Mangosteen Peel Extract Solution. 1. Members. Nathida Wiriyachoke No.2 M.4/1 Chanon Pinyopawasutti No.11 M.4/1 Nuttanon Jantanapornchai No.13 M.4/1 Advisor Dr. Usa Jeenjenkit. 2. Inquiry questions.
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Development of Bleaching Reagent Testing by Using Mangosteen Peel Extract Solution 1
Members NathidaWiriyachoke No.2 M.4/1 ChanonPinyopawasutti No.11 M.4/1 NuttanonJantanapornchai No.13 M.4/1 Advisor Dr. UsaJeenjenkit 2
Inquiry questions • How the mangosteen peel extract solution identify the concentration of bleaching reagent ? • Can mangosteen peel extract identify the concentration of bleaching reagent ? 3
Introduction http://www.vcharkarn.com/varticle/40332 http://www.vcharkarn.com/varticle/42442 http://fic.ifrpd.ku.ac.th/fic/index.php/th/simplelist/434-fruit-28-09-2010.html 4
Bleaching reagent • Sodium Metabisulphite (Na2S2O5) • Sodium hydrosulfite (NaHSO4) • Sodium hypocloride (NaOCl) 5 http://www.palanadenterprises.com/sodium-metabisulphite.htm
The benefit of bleaching reagent • Dying industry : Making new colors • Food industry : making food more attractive 6 http://www.globaltextiles.com/manufacturers/offer-001005099/Others.html
Disadvantage • Frequently use of bleach would effect to the respiratory symptoms (Zock et. al., 2009). http://www.buzzle.com/articles/lower-abdominal-pain-and-lower-back-pain.html 7
One out of 560 larvae treated with BW [bleach solution] (97.3% of the animals were uninfected) was recovered as an adult worm (Zanini & Graeff-Teixeira, 2001). http://www.sciencephoto.com/images/download_lo_res.html?id=903410120 8
Bleach causes a chemical reaction in the gill tissue which blocks the transfer of oxygen and causes the lobster to slowly asphyxiate ( T. Wilson, Vaughan, K. Wilson,Simon & Lockhart , 2007). http://members.cox.net/jjschnebel/lobsnewb.html 9
Scope • Bleaching reagent : sodium metabisulphite • Bleaching reagent concentration :1, 5, 50 and 100 ppm • Dilution ratio ofmangosteen extract solution :1:120, 1:160 and 1:200 10
Mixture ratio of bleaching reagent and mangosteen extract : 1:2 • Wavelength of spectrophotometer : 410 nm 11
Prepare bleaching reagent solution. H2O 1 liter Na2S2O5 0.1 g Na2S2O5(aq) 100 ppm 13
Dilute bleach solution H2O 5 ml Na2S2O5(aq) 5 ml 100 ppm Na2S2O5(aq) 50 ppm 14
Mix the extract solution and different concentration of bleaching solution by 2:1 (v/v). 15
Measure the absorbance by using spectrophotometer at wavelength 410 nm. 16
Results 1:120 of Mangosteen peel extract : water 17
The effect of bleach solution with mangosteen extract Absorbance value Concentration of bleach solution (ppm) 21
Conclusion Mangosteen peel extract solution can identify the concentration of bleaching reagent. 22
Discussion • The color of mangosteen extract solutions were clearer when we put the bleaching solutions. • The higher concentration of bleaching solutions the lower absorbance. 23
Picture shown the mixture of bleach solution and mangosteen peel extract 100 ppm 0 ppm 1 ppm 50 ppm 10 ppm 24
The plots of all ratios between mangosteen extract solution and bleaching solution have the same trend. • We use the absorbance value for drawing graph instead of the others because it is normally use. 25
Benefit • We can use mangosteen extract solution to identify the concentration of bleach reagent by natural substance. 26
Future research • Testing other kinds of bleach reagent such as sodium hydrosulphite instead of sodium metabisulphite. • Develop paper testing for bleaching reagent like Garnier paper. 27 http://poohkiesplace.blogspot.com/2010/08/moisturizers-with-sunscreens.html
References Wilson, D. T., Vaughan, D., Wilson, S. K., Simon, C. N. & Lockhart, K. ( April 2008). Efficacy of a starch-iodine swab technique to detect the illegal use of bleach in a Spiny Lobster (Panulirusargus) fishery. Fisheries Research. Volume 90, Issues 1-3, Pages 86-91. Retrieved from http://www.sciencedirect.com.ejournal.mahidol.ac.th/science/article/pii/S0165783607002494. Zanini, G.M., &Teixeira, C. G.(15 January 2001). Inactivation of infective larvae of Angiostrongyluscostaricensis with short time incubations in 1.5% bleach solution, vinegar or saturated cooking salt solution. Acta Tropic. Volume 78, Issue 1, Pages 17-21.Retrieved from http://www.sciencedirect.com.ejournal.mahidol.ac.th/science/article/pii/S0001706X00001650 28
Zock, J.P. et. al. (October 2009). Domestic use of hypochlorite bleach, atopic sensitization, and respiratory symptoms in adults. Journal of Allergy and Clinical Immunology. Volume 124, Issus 4, Page 731-738. Retrieved from http://www.sciencedirect.com.ejournal.mahidol.ac.th/science/article/pii/S0091674909008744 29
Acknowledgements • Dr. UsaJeenjenkit and our SINOS teachers • Chemistry Department and Biology Department of MahidolWittayanusorn School 30
Thank you for your attention 31