Supplementary Figure 1: Definition of retinoid resistance by morphology, and TALE family gene expres...
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Lan6

Supplementary Figure 1: Definition of retinoid resistance by morphology, and TALE family gene expression response to RA. A) Prototypical RA-sensitive and RA-resistant NB cell lines, treated with DMSO and 13-cisRA. The cell lines LAN-6 and CHLA90 are RA-sensitive cell lines, with smaller cell bodies and some neurite formation in complete media under control conditions (DMSO); when treated with 13-cisRA, proliferation slows and long neuritic processes develop extending from the cell bodies. In contrast, the RA-resistant cells CHLA15 and SK-N-AS have flatter and larger cell bodies under control conditions and no neurite formation or significant apoptosis when treated with 13-cisRA. B) Expression of TALE gene family members other than PBX1 do not correlate with treatment with 13-cisRA. The relative mRNA expression of PBX2, PBX3, PBX4 (top row), MEIS1, MEIS2, MEIS3 (middle row), and PKNOX1 and PKNOX2 in 4 RA-resistant cell lines (blue bars) and 11 RA-sensitive cell lines (red bars), as measured by RT-qPCR.

A

LAN6

CHLA90

CHLA15

SK-N-RA

DMSO

13-cis RA


Supplementary figure 1b see legend above
Supplementary Figure 1B (see legend above) morphology, and TALE family gene expression response to RA.

PBX2

PBX3

PBX4

MEIS1

MEIS2

MEIS3

PKNOX2

PKNOX1


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