Status of susceptibility testing in Aspergillus. Cornelia Lass-Flörl Medical University of Innsbruck, Austria Department of Hygiene and Medical Microbiology AAA 2006 Athens. …the demand and interest for in vitro antifungal susceptibility testing increased. .
Medical University of Innsbruck, Austria
Department of Hygiene and
AAA 2006 Athens
Denning, 1996; Groll 1999; Bodey 1992
……Methods for susceptibility testing
Lack of detection of amphotericin B resistance
Rodriguez-Tudela, 2003; Denning, 1997; Gehrt 1995; Gomez-Lopez 2005
E-test is a commercially available method for antimicrobial susceptibility testing. This technique is based on a combination of the concepts of dilution and diffusion tests.
For Aspergillus spp., good correlations with amphotericin B and itraconazole Etest and M38-A method have been demonstrated.
Espinel-Ingroff 2003; Pfaller 2000; Szekely 1999;Kontoyiannis 2004
MIC is influenced by the choice of growth medium, RPMI-based agars seem to be the most useful.
Ramani 2003; Espinel-Ingroff 1997; Balajee 2002; Lass-Flörl 2001
16 µg/ml Voriconazole
Maiken Arendrup, Denmark
Malcolm Richardson (Executive)
Bertrand Dupont, France
Wolfgang Fegeler, Germany
Francesco Barchiesi, Italy
J Peter Donnelly, the Netherlands (Secretary)
Paul Verweij, the Netherlands (Executive)
Per Sandven, Norway
Juan Luis Rodriguez-Tudela, Spain (Chairman)
Manuel Cuenca-Estrella, Spain
Erja Chryssanthou, Sweden
Jacques Bille, Switzerland (Executive)
Caroline Moore, UK
David Denning, UK (Treasurer)
Cornelia Lass-Floerl, Austria
Aristia Velegraki, Greece
Lynda Fenelon, Ireland
Nikolay Klimko, Russia
…a method to provide a valid, easy, rapid and economic method for testing the susceptibility to antifungal agents of Aspergillus spp.
…to facilitate an acceptable degree of conformity, e.g. agreement within specified ranges and between laboratories in measuring the susceptibility.
Intralaboratory evaluation of the EUCAST-AST-ASPERGILLUS draft. The table summarizes the results of reproducibility per participant. The reproducibility was 0.90 in ICC terms (P<0.01).
Interlaboratory evaluation of the EUCAST-AST-ASPERGILLUS. The table summarizes the results of reproducibility per Aspergillus strain in ICC terms.
Interlaboratory evaluation of the EUCAST-AST-ASPERGILLUS. The table summarizes the ranges and percentages of MIC values (µg/ml)
The method differentiated amphotericin B or itraconazole-resistant Aspergillus strains in vivo from the susceptible ones. The MICs of amphotericin B and itraconazole were > 2 and > 8 µg/ml, respectively.
Denning 1997; Diaz-Guerra 2003; Osherov 2001; Chen 2005; Oakley, 1997