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Conserve Fibroblast growth factor 8 (fgf 8) domains

Conserve Fibroblast growth factor 8 (fgf 8) domains. Ana Tomas Judith Paridaen Susana Domingues. Member of the FGF family. Function: Mitogenic Chemotactic Angiogenic activity Promoting cell growth, differentiation and motility. Fibroblast growth factor 8. N.º of a.a.: 233

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Conserve Fibroblast growth factor 8 (fgf 8) domains

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  1. Conserve Fibroblast growth factor8 (fgf 8) domains Ana Tomas Judith Paridaen Susana Domingues

  2. Member of the FGF family Function: Mitogenic Chemotactic Angiogenic activity Promoting cell growth, differentiation and motility Fibroblast growth factor 8

  3. N.º of a.a.: 233 Molecular weight:26525.4 Theoretical p.i.:10.44 Total n.º of negatively charged residues (Asp + Glu):19 Total n.º of positively charged residues (Arg + Lys):40 Formula:C1164H1877N363O330S9 Total n.º of atoms:3743 Grand average of hydropathicity: -0.651 (soluble) Estimated half-life: The N-terminal of the sequence considered is M (Met) The estimated half-life is:  30 hours (mammalian) Instability index: The instability index (II) is computed to be 47.80 Unstable protein Some Fgf 8 characteristics ProtParam tool - ExPASy

  4. Structure • 6 chains present • 913 residues

  5. SP binding HBGF/FGF Structure and active sites ProSAT - Protein Structure Annotation Tool

  6. Axolotl Chicken Ciona S. Cow Dog Drosophila Fugu Houseshrew Human Japanese lamprey Mouse Opossum Oryzias Rabbit Rat Rhesus macaque Shell turtle Tetraodon Zebrafish Models Ciona

  7. Phylogenetic tree Clusteralgorithm

  8. ErkDD Kin_bind RDCIFTEIVLENNYT p85_SH2 SH2KGAETGFYICMNKRG InsR_Kin Y_kin NARYEGWYMAFTRRG Consensus domains Multalin – Multi alignment program

  9. ErkDD Kin_bind RDCIFTEIVLENNYT p85_SH2 SH2KGAETGFYICMNKRG InsR_Kin Y_kin NARYEGWYMAFTRRG 3D structure and consensus domains Rasmol

  10. Receptor Fgf Fgf8 structure with receptor Rasmol

  11. Common regulatory elements in Fgf8 downstream targets Anna Ferrer-Vaquer Alisa Fuchs

  12. Fgf8 signaling pathway Elk Ets cJun Fos Myc ...

  13. Fgf8 inducible genes • Sprouty1 > Mm, Gg, Dr • Pax3 > Mm, Gg, Dr • Otx2 > Mm, Gg, Dr • Tbx2 > Mm, Gg, Dr • Erm • Pea3 • Ptx1 • Lhx6 • Sef • AP2 • Flrt3 .... Many more • Sprouty1 • Pax3 • Otx2 • Tbx2 • Erm • Pea3 • Ptx1 • Lhx6 • Sef • AP2 • Flrt3 .... Many more Limitation: 1Kb of 5’UTR

  14. Alignment Alignment of sequences from different genes to find consensus blocks Not reliable

  15. Promoter analysis

  16. Results of promoter analysis

  17. Conclusion Analysis of nucleotide sequences is much more difficult than amino acids

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