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Chapter 1—Main Themes in Microbiology. Material on Midterm Exam. Chapter 1 Outline. Safety & Dirty Spot lab Dirty Spot Results—scope of Micro 1.2-1.4 Discussion & Summary Handwashing Lab Graphing Quiz-size/scope-results Scientific Method. Aseptic Technique & Pasteur ideas

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Presentation Transcript
chapter 1 outline
Chapter 1 Outline
  • Safety & Dirty Spot lab
  • Dirty Spot Results—scope of Micro
  • 1.2-1.4 Discussion & Summary
  • Handwashing Lab
  • Graphing Quiz-size/scope-results
  • Scientific Method
  • Aseptic Technique & Pasteur ideas
  • Biogenesis Lab setup
  • Ubiquitous lab setup
  • Review
  • Test
  • Ubiquitous results
process box 1 1
PROCESS BOX 1-1
  • Based on the information on the previous slide, work with your table and come up with a formal definition of microbiology

MUST BE AT LEAST 3 LINES

formal definition
Formal Definition
  • Microbiology is a special area of biology that deals with tiny life forms not readily observed without magnification
general characteristics of microorganisms
General Characteristics of Microorganisms
  • Organization
    • Prokaryotes v. eukaryotes
  • Size
    • 10nm-3mm
  • Lifestyles
    • Parasites v. free-living
  • All living?
    • Viruses
process box 1 2
PROCESS BOX 1-2
  • Even though we will be studying viruses in this course, why won’t we be able to “see” them?

MUST BE AT LEAST 2 LINES

slide8

Aseptic Technique

  • Wash hands! (20s lather, 20s rinse)
  • Wipe down lab bench
  • Conduct lab
    • Flame all glass containers when opened and before closed
    • Flame loops before and after transfer (but let cool!)
  • Wipe down lab bench
  • Wash hands! (20s lather, 20s rinse)
cornell notes
Cornell Notes
  • Method of note taking
  • You will do for Sections 1.2-1.4 in text
slide10

With groups, talk about HW and agree on 3-5 key points per section

  • You will then be assigned ONE OF THE THREE sections
slide11

Student 1

Student 2

Section ______ Summary

Student 3

Student 4

the fall of superstition and the rise of microbiology
The Fall of Superstition……and the Rise of Microbiology
  • Spontaneous Generation
  • Biogenesis
  • Abiogenesis
  • Francesco Redi
  • Louis Jablot
  • Franz Shultze/Theodore Schwann
  • Louis Pasteur
process box 1 3
PROCESS BOX 1-3
  • Why do you think Jablot and Pasteur’s closed infusions turned out to be sterile, unlike those of several other experiments?

MUST BE AT LEAST 2 LINES

scientific method
Scientific Method
  • Key terms
    • Independent variable
    • Controlled variable
    • Control
    • Dependent variable
    • Hypothesis
    • Theory
    • Law
antonie van leeuwenhoek
Antonie van Leeuwenhoek
  • Contribution: the microscope
  • Called father of bacteriology, protozoology
slide20

“In the said matter there were many very little animalcules, very prettily a-moving…Moreover, the other animalcules were in such enormous umbers, that all the water…seemed to be alive”

john tyndall ferdinand cohn
John Tyndall & Ferdinand Cohn
  • Tyndall
    • Some microbes have high heat resistance
  • Cohn
    • Discovered Endospores (heat-resistant structures)

Concept of sterility—completely free of ALL forms of life

slide22

Aseptic Technique: process of handling microbes in a way that prevents infection of handler and others exposed

Robert Koch

Joseph Lister

Aseptic Technique

Oliver HolmesIgnazSemmelweis

robert koch
Robert Koch
  • Koch’s Postulates
    • Proofs that verified germ theory
    • Could establish if an organism was pathogenic and which disease it caused
    • Bacillus anthracis

THIS is how you test for pathogenicity!

classification
Classification
  • Orderly arrangement of organisms that indicate evolutionary relationships and history
  • DKPGOFGS
  • Based on
    • Morphology
      • Structure
    • Physiology
      • Function
    • Genetics
      • Inheritance
what s in a name
What’s in a name?

KNOWING LATIN AND GREEK WORD PARTS IS HUGE!

process box 1 31
PROCESS BOX 1-3
  • Using knowledge of Latin/Greek prefixes, predict some information about Helicobacter pylori and Thermusaquaticus.

MUST BE AT LEAST 3 LINES