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Lux System Constructs

This document details the construction phases of several sender and receiver constructs in the Lux system, specifically focusing on AHL (N-acyl homoserine lactone) based signaling. Includes information on sender constructs with LVA+ (increased degradation rate) and LVA- (normal degradation rate), the testing of receiver components, and the integration of reporter genes such as YFP and eCFP. Updates on the construction status, including missing components and modifications, are provided, along with notes on the propagation capabilities of these genetic constructs.

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Lux System Constructs

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  1. Lux System Constructs Sender Construct (LVA+): aTc -> AHL Receiver Construction Intermediate: Receiver Output Component (Weiss): AHL -> YFP, cI repressible Sender Construct (LVA-): aTc -> AHL Sender Reporter (eCFP), Ptet Test Receiver Output+Propagation Component: AHL-> {YFP, AHL}, cI repressible, LVA+ Receiver Test Construct: AHL -> YFP Receiver Output+Propagation Component: AHL-> {YFP, AHL}, cI repressible, LVA- Receiver Repressor Component: AHL -> cI

  2. Lux System Constructs: Status Lig./Trans. Today Done Construct Status Notes Sender Construct (LVA+): aTc -> AHL Completed J06001 Building F1611 missing. (R0040+B0034) + (C0161+B0015) Sender Construct (LVA-): aTc -> AHL J06002 Completed Sender Reporter (eCFP), Ptet Test J06003 Receiver Test Construct: AHL -> YFP Building I13272 missing. (R0063+I0462) + (R0062+E0432) J06000 Receiver Repressor Component: AHL -> cI Building F2621 initially missing. (R0063+I0462) + (R0062 + B0030) + (C0051+B0015) J06004.x (vary RBS)

  3. Lux System Constructs: Status Construct Status Notes Building Receiver Output Construction Intermediate: (R0065+B0034) + E0032 J06006 Building (R0065+B0030)+ (E0032 + B0015) Receiver Output Component (Weiss): AHL -> YFP, cI repressible Building Receiver Output+Propagation Component: AHL-> {YFP, AHL}, cI repressible, LVA+ Building Receiver Output+Propagation Component: AHL-> {YFP, AHL}, cI repressible, LVA- Building

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