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Electrospinning is a simple and cost-effective technique, it can fabricate biomimic fibrous membranes under a high voltage electrostatic field, and the obtained fibers range is from several nanometers to submicron level in diameter). The electrospun extracellular matrix (ECM)-like architecture of biomimic fibrous membranes were widely applied in tissue engineering. In present research, a biodegradable polymer of poly(γ-glutamic acid)(γ-PGA) was successfully electrospun by the dimethyl sulfoxide(DMSO) aqueous solution(the ratio of DMSO：H2O = 70：30, and the concentration of γ-PGA = 25wt%) to fabricated biomimic fibrous membranes. The average diameter of obtained fibers were in the range from 300nm to 1μm. The electrospun mats of γ-PGA were quickly humidified at room temperature due to highly humidity with the large surface area and high porosity. For further research, mouse embryonic fibroblast cell line(NIH3T3) were used to perform the biocompatibility of γ-PGA’s membranes. The results show the matrices of γ-PGA’s biomimic membranes can promote the cell adhesion(average cell numbers was 1.5 times more than tissue culture polystyrene) and cell proliferation(average cell numbers was 7 times more than tissue culture polystyrene). The degradation time and mechanical properties of these biomimic fibrous membranes can be easily prolonged and enhanced by treatment with cross-linking agent of 1-3-dimethyl-aminopropyl-3-ethylcarbodiimidehydrochloride(EDC), The 25wt% γ-PGA’s DMSO aqueous solution by electrospinning could fabricate microfibrous mats and it can be applied as a suitable ECM-like environment for cell sheet engineering use in the near future.