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This study investigates the phase contrast microscopy and immunofluorescence profiling of various antigens, including CD31, vWF, CD133, and VEGFR in diluted LDL samples. Using specific dilutions and stains like PE and FITC, we analyze the expression levels of these markers crucial for understanding vascular biology and endothelial function. The findings contribute to the understanding of how LDL interactions with vascular cells impact health and disease, particularly in conditions related to vascular inflammation and pathology.
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B B A A phase contrast Dil - Ac - - LDL D D C C CD31 CD31 vWF vWF E F F 3 10 1000 PE PE 2 10 H H - - - - 1 10 SSC SSC CD133 CD133 0 10 0 0 0 0 1 1 1 2 2 2 3 3 3 4 4 4 0 1 2 3 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 VEGFR VEGFR - - 2(KDR) 2(KDR) CD34 CD34 - - FITC FITC