1. Mailing list The hospital sends a Patient information mailer to all surgical patients. Select patients get a Tissue Bank Brochure. This happens about two weeks before surgery.

2. Pre-admission Testing Schedule Most patients come in for pre-admission work up usually within a week of surgery. The patients identified in blue are patients that received a Tissue Banking Brochure

3. Pre-admission Testing Schedule Most patients come in for pre-admission work up usually within a week of surgery. The patients identified in blue are patients that received a Tissue Banking Brochure

4. Daily Surgical Schedule Patients that were approached will be cross referenced with the daily surgical schedule and appropriately color coded

5. Tissue Banking Operations • Specimen tracking • Specimen tracking form • Communication with operating room: “Navicare” • Timely transfer of tissue from the OR to the Pathology Department (Tissue is sent up like a frozen section)

10. Tissue Banking Operations • Selecting and Handling Tissue • Assessment of tissue / Pathologist review • Processing of tissue • Size / format / “matched” samples • Freezing techniques • Liquid nitrogen • Vapor phase of “liquid” nitrogen (Cryoshipper) • - 80 C freezer • Within the cryostat (-20 C) • Dry Ice

11. Liquid nitrogen vapors for snap-freezing Approx. -190°C Refilled once a week Cryoshipper

12. Tissue Banking Operations • Formats of tissue • Frozen (with or without OCT) • Fresh tissue (Media requirements) • Paraffin embedded tissue Matched to Frozen samples TMA’s (3-5 mm thick sections suitable for tissue micro arrays) Archived pathology blocks / unstained sections Paraffin curls (10 - 20 um sections • Cytology Samples (Scraps and fluids) • Blood (whole blood, serum, plasma) • Core Biopsy of small tumors

13. Frozen Vials Normal & abnormal vials can be designated as such w/different colored caps or by manipulating barcodes.