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第39章 细胞代谢与基因表达调控

第39章 细胞代谢与基因表达调控. ,. 乳糖操纵子调节机制. (一)乳糖操纵子的结构 诱导型操纵子 P -- RNA 酶结合的 DNA 序列 -10区 TATAAT 和-35区 TTGACA, 强启动子 O -- 结合阻遏物,是 RNA 酶能否通过的开关. (二)、阻遏蛋白的 负性 调节 无乳糖 lac 操纵子处于阻遏状态 有乳糖 lac 操纵子即可被诱导 诱导剂 半乳糖 IPTG ( 三) CAP 的 正性 调节 CAP— 分解代谢基因激活蛋白 同二聚体 DNA 结合区 cAMP 结合位点.

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第39章 细胞代谢与基因表达调控

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  1. 第39章细胞代谢与基因表达调控

  2. 乳糖操纵子调节机制 • (一)乳糖操纵子的结构 诱导型操纵子 • P-- RNA酶结合的DNA序列 -10区TATAAT和-35区TTGACA, 强启动子 • O-- 结合阻遏物,是RNA酶能否通过的开关

  3. (二)、阻遏蛋白的负性调节 • 无乳糖 lac操纵子处于阻遏状态 • 有乳糖 lac操纵子即可被诱导 • 诱导剂 半乳糖 IPTG • (三)CAP的正性调节 • CAP—分解代谢基因激活蛋白 • 同二聚体 • DNA结合区 • cAMP结合位点

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