1 / 16

Portable SPR instrument: From research to clinical applications

Portable SPR instrument: From research to clinical applications. Jean-François Masson Université de Montréal Photonics North 2014 – Parlons Affaires. Field of the innovation. Scientific equipment for the research , industrial applications and clinical diagnostics Innovation:

risa
Download Presentation

Portable SPR instrument: From research to clinical applications

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Portable SPR instrument: Fromresearch to clinical applications Jean-François Masson Université de Montréal PhotonicsNorth 2014 – Parlons Affaires

  2. Field of the innovation • Scientificequipment for the research, industrial applications and clinical diagnostics • Innovation: • Small and portable SPR instrument • Surface chemistry to analyzecrudebiofluids • Nanomaterial to amplifyresponsefrom instrument

  3. Commercial SPR instrument – lab-based • Pros: High sensitivity, reliability and automation • Cons: High cost of acquisition, operation and maintenance, not possible to use biofluids

  4. Commercial SPR instrument – Portable • Pros: portability, low acquisition cost • Cons: Poorer performance, maintenance, user-friendliness, low number of sensing channels (typically 1-2)

  5. Integrated portable SPR instrument Features • Sensitive • 10-6 RIU resolution • Computer poweredwith USB – no external power cord • No RI matchingfluids • Simple injection system • 4-channels including a reference • Simplicity • Versatility • Performance • Portability • Customizable • Low maintenance • Lowcost • Lowweight (lessthan 1.3 kg) • Quiet operation

  6. Industrial application – Salinitymeasurement Reproducibility < 1% and thechannel to channel variation < 10% • Calibration runwith 3 channels (A-C active), background subtractedwithchannel D • Triplicaterepeat of calibration point 2 at the end of the run R2 = 0.9998 % CV = 0.6 to 8% Repeatability: 0.4 % (6.02 ± 0,02) nm

  7. Screening growth hormone-releasing peptides (GHRPs) Type B scavenger receptor CD36 (CD36: cluster of differenciation 36) • EP80317: anti-atherosclerotic property mediated by CD36 • interfering with the binding of oxLDL to the scavenger receptor expressed on macrophages

  8. Therapeutic drug monitoring Methotrexate – chemotherapy agent • Drugswith a narrowtherapeuticwindow must becarefullycontrolled to ensurepatient’ssafety, propermetabolism and drugefficacy • Common analyticaltechniques are laboratory-based, time-consumingor expensive to run • The development of a smalland portable instrument with a simple and dedicated set of reagentswouldaddresssome of the current challenges in TDM

  9. Quantification of MTX in clinicalsampleswith SPR instrument Collaboration with a local hospital to analyzeactualclinicalsamples • The samplesweredeidentified and analyzed in a blindassaywith FPIA (independantanalyst) and with LC-MS/MS and SPR (differentanalyst SZ) • FPIA analysisresultswereonlyknownafter final report from SPR and LC-MS/MS

  10. Calibration fromdifferentoperators and locations Calibrations wererun in UdeMlaboratories and at local hospital • The system wasdeployedatHosp. Maisonneuve-Rosemont to evaluate the performance in a real environment • The SPR system is running on power supplied by the portable computer • Ease of portability 10 uM Day 1 CV = 4-8% 0 uM Day 2 CV = 6-16% 50 uM Day 2 CV = 9-23%

  11. Acute lymphoblasticleukemia • Measuring the immune responseduringtreatment • Some patients respond to asparaginasetreatment by developingantibodies, leading to ineffective treatment and allergicreactions • Monitor antibody concentration by immolizing the drug (asparaginase) on the SPR sensor His-tag Asparaginase SPR sensor

  12. Proteinbiomarkers • Prostate-specificantigen (PSA) Screening method: • Digital rectal exam: • Low cost • Unpleasant • Only screen a section of the prostate • Prostate specific antigen (PSA) test: • Protein secreted by the prostate cells • Level measure in a blood sample • Complementary to digital rectal exam foran accurate diagnostic ***Ratio of PSA + Ab-2 (1:1) used LD: 0,1 nM

  13. Interfacingwithotherspectroscopic techniques • SPR-fluorescence Multi-channel SPR and fluorescence instrument: • SPR and fluorescence are orthogonal techniques thatcanbecombined in a small instrument Fluorescence Fluorescence SPR SPR

  14. Proof-of-principlewith SPR-fluorescence bioassay • Fluorescent antibodyused: Cy3 Anti-Human-IgG • Usingmicrostructured gold film couldimprove the fluorescence emission Positive control (1000nM IgGimmobilized) SPR Fluorescence

  15. Differentmarkets accessible • Research (ready to market) • Ligand screening • Bindingassays • Optical research / Surface-enhanced spectroscopies • Industrial applications (partiallyvalidated) • Refractive index measurement • Fermentation processes • Clinical diagnostics (in process ofvalidation) • Biomarkers • TDM

  16. Acknowledgements Currentresearch group: Dr. Natalia Bukar Dr. Hélène Yockell-Lelièvre Dr. KristyMcKeating Dr. Marc Vidal Sandy Shuo Zhao * Julien B.-Turcot Maxime Couture Hugo-Pierre P-Richard Rita Faid Alexandra Aubé Hu Zhu Simon Forest Daniel Pelechacz * Félix Lussier Jérémie L.-Carbonneau Geneviève Granger Gabrielle L. Lajoie Corentin Geny * Alumni

More Related