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Wild type. Kir Mice. ORNs. G. MC. ORNs. G. MC. Quantify the tuning curve shape by computing S (Sparseness) Index . (Michelle L Schlief & Rachel Wilson, 2007). Interval. Water Delivery. Water Port IR Cross. Two-choice Test. Initial Training

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Presentation Transcript
slide1

Wild type

Kir Mice

ORNs

G

MC

ORNs

G

MC

slide2

Quantify the tuning curve shape by computing S (Sparseness) Index.

(Michelle L Schlief & Rachel Wilson, 2007)

two choice test

Interval

Water Delivery

Water Port IR Cross

Two-choice Test
  • Initial Training
  • Motivation to water- Training water-deprived (about 1.5 ml per day) mice.
  • Mice familiar with the boxes.
  • Interest to holes- Mice were rewarded with water for every nose poke into the sniff port.
  • Training 1- Associate the odor with the water port.
  • a. Odor A with water A.
  • b. Odor B with water B.
  • 5. Two choice

WaterA

Odor

WaterB

3.

slide4

Methyl Butyrate

Methyl Valerate

E-2

E-3

E-4

E-5

E-6

slide5

E-1 E-2 E-3 E-4 E-5 E-6 E-7 E-8 E-9 E-2

Wt (normal projection) mice show better performance than Het (disrupted projection) at some concentration.

cross habitation
Cross-habitation

Odor presenting sequence:

MO MO MO MO MO OdorA OdorA OdorA OdorA OdorA OdorB OdorB

Waiting time = 5 min, Odor presenting duration = 2 min, Interval = 3 mins

Flow rate: total: 300ml/min each box, 30ml/min for odor deliver each box.

slide9

Amyl Acetate

Vs Heptanal

B6, 12mice

slide11

Amyl Acetate

Vs Heptanal

WT/HET

9 animals

slide12

Amyl Acetate vs Heptanal

HET/HET

9 animals

slide20

Mixture

Dilution

Amyl Acetate vs. Heptanal