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HPLC Bulk Property Detectors PowerPoint Presentation
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HPLC Bulk Property Detectors

HPLC Bulk Property Detectors

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HPLC Bulk Property Detectors

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  1. HPLC Bulk Property Detectors Refractive Index Detector

  2. HPLC Bulk Property Detectors Refractive Index Detector Plus: 1) Measures a bulk property 2) Nearly Universal (different RI than mobile phase) 3) Comparable response for different analytes 4) Detects species with no chromophores Minus: 1) Temperature dependent 2) Poor sensitivity (LOD ≈ 100 ng) 3) No gradient elution

  3. HPLC Bulk Property Detectors Evaporative Light Scattering Detector Solid state laser

  4. HPLC Bulk Property Detectors Evaporative Light Scattering Detector Plus: 1) Measures a bulk property 2) Nearly Universal (must be non-volatile) 3) Does not detect liquids (gradients are ok) 4) Detects species with no chromophores Minus: 1) Signal not linear with concentration 2) Fair sensitivity (LOD ≈ 1 ng) 3) No salts or buffers in mobile phase

  5. HPLC Bulk Property Detectors Conductivity Detector

  6. HPLC Bulk Property Detectors Conductivity Detector Plus: 1) Measures a bulk property 2) Nearly Universal (must be ionic) 3) Common for Ion Exchange LC 4) Detects species with no chromophores 5) Simple, robust Minus: 1) Fair sensitivity (LOD ≈ 1 ng) 2) No salts or buffers in mobile phase 3) Gradients a challenge

  7. HPLC Specific Property Detectors Conventional UV-Vis Absorption Detector Photodiode 1 Photodiode 2 Hg Pen Lamp 254 nm

  8. HPLC Specific Property Detectors Multi-wavelength UV-Vis Absorption Detector Deuterium Lamp Photodiode Array

  9. HPLC Specific Property Detectors Multi-wavelength UV-Vis Absorption Detector

  10. HPLC Specific Property Detectors Multi-wavelength UV-Vis Absorption Detector Plus: 1) Measures a specific property 2) Nearly Universal (must have chromophore) 3) Most common of all detectors (~75%) 4) Potential to provide qualitative info. 5) Simple, robust Minus: 1) Fair sensitivity (LOD ≈ 1 ng) 2) Expensive with PDA, limited with Hg Lamp 3) Misses some important analytes

  11. HPLC Specific Property Detectors Fluorescence Detector

  12. HPLC Specific Property Detectors Fluorescence Detector

  13. HPLC Specific Property Detectors Fluorescence Detector Plus: 1) Measures a specific property 2) Highly Selective (must fluoresce) 3) Second most common of all detectors (~15%) 4) High sensitivity (LOD ≈ 0.01 ng) 5) Can interrogate very small volumes Minus: 1) Not Universal 2) Limited Applications

  14. HPLC Specific Property Detectors Electrochemical Detector

  15. HPLC Specific Property Detectors Electrochemical Detector Plus: 1) Measures a specific property 2) Highly Selective (depends on reduction potential) 3) High sensitivity (LOD ≈ 0.01 ng) Minus: 1) Not Universal 2) Must have electrolyte in mobile phase 3) Mobile phase must be aqueous 4) Gradients not possible

  16. HPLC Element Specific Detectors ICP Detector Detector

  17. HPLC Element Specific Detectors ICP Detector Detector Plus: 1) Can be Highly Selective 2) Can be nearly Universal 3) Can have single sensitivity for all analytes Minus: 1) $$$ 2) Mobile phase must be aqueous 4) Mobile phase must not contain element of interest

  18. UV-Vis detection of sugars in juice

  19. UV-Vis detection of sugars in juice

  20. ICP Carbon detection of sugars in juice

  21. ICP Carbon detection of sugars in orange juice

  22. ICP Carbon detection of amino acids

  23. ICP Carbon detection of amino acids

  24. HPLC Derivatization Detectors Tag analytes with fluorophore Before or After Separation

  25. HPLC Derivatization Detectors Tag analytes with fluorophore Before or After Separation

  26. HPLC Indirect Detectors Detect the change in a constant signal as the analyte passes

  27. Recent HPLC Detector Hollow Cathode Detector

  28. Recent HPLC Detector Hollow Cathode Detector

  29. Recent HPLC Detector Hollow Cathode Detector

  30. Recent HPLC Detector Hollow Cathode Detector

  31. Recent HPLC Detector Hollow Cathode Detector

  32. Recent HPLC Detector Hollow Cathode Detector