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+TOF Product (461.8): Experiment 2, 22.717 to 22.756 min from w203_ALBU_BOVIN_digest....

+TOF Product (461.8): Experiment 2, 22.717 to 22.756 min from w203_ALBU_BOVIN_digest. Max. 192.3 counts. a=3.55491270775816470e-004, t0=6.56947286939503100e+001. 190. 173.13. 461.8 (2+) AEFVEVTK. 180. a2. 170. 160. 150. y6 O means y6-H 2 O. 140. 130. K. 120. T. V. 110. V.

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+TOF Product (461.8): Experiment 2, 22.717 to 22.756 min from w203_ALBU_BOVIN_digest....

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  1. +TOF Product (461.8): Experiment 2, 22.717 to 22.756 min from w203_ALBU_BOVIN_digest.... Max. 192.3 counts. a=3.55491270775816470e-004, t0=6.56947286939503100e+001 190 173.13 461.8 (2+) AEFVEVTK 180 a2 170 160 150 y6O means y6-H2O 140 130 K 120 T V 110 V E b2 F E 100 Intensity, counts 201.14 90 A 80 70 a2O F 155.12 60 y6 120.11 50 722.47 y2 y5 40 E y4 y3 y1 248.18 30 376.26 102.08 V b1 476.28 575.38 147.15 347.28 20 219.18 a3 y4O y6O y7 y2O b4 y5O y7O 72.11 10 330.23 851.75 447.2 229.14 704.53 557.38 833.51 0 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 m/z, amu

  2. 740.4 (2+) Whole spectrum LGEYGFQNALIVR I/L N Q A Y F E G y9 y5 y6 y7 y4 y10 y11 y8 1309.7

  3. b2 [VA] or [GX] 740.4 (2+) MH+=1479.8 LGEYGFQNALIVR LOW MASS REGION Y R b3 V I/L y1 I/L y2 F I/L E y3 (W) (V)

  4. 582.3 2+ = 1164.6 immonium : X, V, 147,129 -> Lys C term 1164-1050 = 113 X ? -> 951 99 V -- b ion at 213 – ion minus NH3 at 934 -> 837 114 N -- b ion at 327 -> 708 129 E --b ion at 456 (weak) -> 595 113 X -- b ion at 569 -> 494 101 T -– ion minus H2O at 934 -> 365 129 E or -> 357 137 H ? but no 110, so no His -> 365-147=218 : could be MS or FA MARK peak at 218 ! 147+87=234 – no peak there 147+131=278 – no peak there 147+147=294 – no peak there 147+71=218 – weak but clear peak there => XVNEXTEFAK **************************************************** 740.4 2+ immoniums : X, V, F,Y,E 1309-1180 : 129 E 1180-1017 163 Y -17 NH3 loss ->960 57 G ->813 147 F -17 NH3 loss -> 685 128 Q (K) no NH3 loss any more -> 571 114 N -> 500 71 A -> 387 113 X ( ->300 ? 87 Ser, but the 300 peak is a B (complem. To 1180 ) -> 274 113 X -> 175 99 V so : …EYGFQNAXXVR missing : 1439-1309 : 170 = VA or XG – visible as 171 protonated mass typical problem: n-term dipeptide ambiguity [XG] EYGFQNA(VR or [VA] EYGFQNALIVR …..FGTSAE….K [VG]FGTSAE????K msms spectra analysis 461.8 (2+)  MH+ = 922.6 immonium : V,F,E Y1 = 147, no 175, thus K start 722.47 575.38 = 147 F 476.28 = 99 V  347.28 = 129 E  248.18 = 99 V  147.15 = 101 T extend upstream : 722  851 = 129 E 922-851 **** 464.2 x 2 = 928.4 -1 = 927.4 (MH+) 927->764 : 163 Y ( tyr immonium at 136 ! ) -> 651 : 113 I/L ->488.23 : 163 : Y ->359.24 : 129 E -> ? nothing clear look at other things : 175 : R 928-651=277 = YL 277-249=28 carboxy loss from a b ion last y ion : 359 -> 175 : 184 missing dipeptide ions masses (! Lists values + 1 proton) 185 : PS or AL P : probably not in this peptide (no 70 peak) thus AL 359-71=288 nothing there 359-113=246 weak but clear peak there -> YXYEXAR *******************************

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