herpesviruses
Download
Skip this Video
Download Presentation
Herpesviruses

Loading in 2 Seconds...

play fullscreen
1 / 41

Herpesviruses - PowerPoint PPT Presentation


  • 144 Views
  • Uploaded on

Herpesviruses. September 9,14, 2010. Shanthi and Kumari, December 1993 –April 1995. Smithsonian National Zoological Park, Washington. The elephant herpesvirus Richman et al. 1999. Science 283:1171. The case of the elephant herpesviruses (Richman et al. 1999. Science 283:1171).

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'Herpesviruses' - remy


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
herpesviruses

Herpesviruses

September 9,14, 2010

shanthi and kumari december 1993 april 1995
Shanthi and Kumari, December 1993 –April 1995

Smithsonian National Zoological Park, Washington

the case of the elephant herpesviruses richman et al 1999 science 283 1171
The case of the elephant herpesviruses (Richman et al. 1999. Science 283:1171)

Fatal hemorrhagic

Disease in Asian elephants

African elephant herpesvirus

(“cold sores”)

Asian elephant herpesvirus

(“cold sores”)

Fatal hemorrhagic

Disease in African elephants

viruses with ds dna genomes
Viruses with ds DNA genomes

Papovaviridae

bovine

herpesvirus-1,2

equine

herpesviruses -1,4

Adenoviridae

porcine

cytomegalovirus

Herpesviridae

malignant catarrhal

fever virus

Poxviridae

African swine fever virus

herpesviridae
Alphaherpesvirinae(BHV-1, EHV-1,4, FelineHV, CanineHV)

Grow rapidly

Latency in sensory neurons

Betaherpesvirinae

Cytomegaloviruses (large balloon-like cells)

Grow slowly

Latency in salivary glands, kidneys, lymphocytes

Gammaherpesvirinae(malignant catarrhal fever virus)

Lymphoproliferative diseases

Latency in lymphoid cells

Herpesviridae
structure
Structure

nucleocapsid

envelope

tegument

glycoproteins

(gB, gC…gL)

slide9
Latency and Reactivation

Viral DNA

IE genes

(regulatory)

E genes

(enzymes)

L genes

(structural)

LAT

reactivation

stress

immunosuppression

corticosteroids

cAMP

bhv 1 ibr virus
Infectious bovine rhinotracheitis

Disseminated fatal disease of newborns

Abortions

Genital lesions

Encephalitis

BHV-1(IBR virus)
infection in the animal
establishment of

latency

(infection for the first time)

Infection in the animal

Infection of

replication in

reactivation

susceptible animal

epithelial cells

from

latency

replication in

epithelial cells

(rhinotracheitis)

systemic

cell-associated

spread

infection of the fetus

encephalitis

-> abortion

complications of bhv 1 infection
abortions

encephalitis

fatal disease in newborn calves

Shipping fever (M. hemolytica, P. multocida)

Bronchopneumonia -> fibrinous pleuropneumonia

Complications of BHV-1 infection
factors contributing to shipping fever
environmental

crowding animals from different sources

stress

host-virus

paralysis of mucociliary escalator

release of iron and nutrients

increased colonization

immunosuppression

Factors contributing to shipping fever
diagnosis of bhv
virus isolation

immunofluorescence

immunohistochemistry

PCR

serology

virus neutralization

ELISA

Diagnosis of BHV
vaccination against bhv 1
modified-live (attenuated)

intra nasal

intra muscular

inactivated

intra muscular

Vaccination against BHV-1
equine alpha herpesviruses
Equine alpha herpesviruses

5 other known herpesviruses

ehv 1 and ehv 4
Immunologically related

Respiratory disease

EHV-1causes abortions, neurological disease

Immunity short lived

Reinfection or reactivation

EHV-1 and EHV-4
ehv 1 and ehv 4 respiratory disease
Incubation period (1-10 days)

Primary infection in young horses

Biphasic fever

Nasal discharge, cough

Uncomplicated cases -> complete recovery 1-2 weeks

EHV-1 and EHV-4 (respiratory disease)
ehv 1 associated disease
EHV-1 associated disease

Acute respiratory disease in young horses

Abortions (3rd trimester but can also occur at other times)

Myeloencephalopathy (EHM)

myeloencephalopathy by ehv 1
often but not always associated with respiratory disease

often several horses

sudden onset rapid progression, early stabilization

ataxia, paresis, urinary incontinence, cystitis

little evidence of viral replication in neural tissues (immune mediated?)

vasculitis, thrombosis, hemorrhages

(See EHV-1 Consensus Statement. J Vet Int Med. 2009. 23:450-461)

Myeloencephalopathy by EHV-1
risk factors for ehv 1 myeloencephalitis
Risk factors for EHV-1 myeloencephalitis
  • Viral (Nugent et al. 206. J. Virol 80:4047
    • N752D mutation
  • Host (Goehring et al. 2006. J. Vet. Int. Med. 20:601)
    • sex - female
    • age - > 3 yrs
    • season - fall, winter
    • fever
    • breed - Hispanic, Standardbred, Draught
slide25
Goodman et al. 2007. A point mutation in a herpesvirus polymerase determines neuropathogenecity. PLoS Pathogen. 3(11):e160.doi.1371
viraemia by neuropathogenic and non neuropathogenic ehv 1
Viraemia by neuropathogenic and non-neuropathogenic EHV-1

Allen and Breathnach 2006. Eq Vet Jn 38:252

ehv 1 abortions
third trimester most likely

few weeks to several months after respiratory outbreak

abortion storms

EHV-1 abortions
pathogenesis of abortion
Pathogenesis of abortion

viraemia

endometrial endothelial cell infection

endometrial vasculitis and thrombosis

extensive infarction:

virus negative fetus

pathogenesis of abortion29
Pathogenesis of abortion

viraemia

endometrial endothelial cell infection

endometrial vasculitis and thrombosis

extensive infarction:

virus negative fetus

limited infarction:

virus positive fetus

prevention of ehv 1 disease
management

vaccines

short-lived immunity (mares - 5,7,9 months of gestation)

reduce severity but do not prevent

no vaccines licensed to prevent encephalomyelitis

Prevention of EHV-1 disease
diagnosis
Diagnosis
  • Virus isolation
  • PCR
    • Type 1 vs 4
    • D752 vs N752
  • serology
other herpesviruses
Other herpesviruses
  • alpha herpesviruses
    • porcine herpesvirus 1 (pseudorabies/Aujeszky’s disease)
    • Canine herpesvirus
    • Feline herpesvirus
    • Marek’s disease
  • beta herpesviruses
    • porcine cytomegalovirus
  • gamma herpesviruses
    • malignant catarrhal fever
porcine herpesvirus
porcine herpesvirus
  • endemic in most parts of the world - Canada considered free (reportable disease)
  • wide host range
  • pigs
    • asymptomatic
    • abortions
    • fatal disease in new born piglets
    • respiratory, neurological
  • other species
canine herpesvirus
canine herpesvirus
  • wide-spread, usually asymptomatic
  • generalized fatal disease in puppies (fading puppy syndrome)
  • Ocular (Ledbetter, 2009. Vet Ophthalmol. 12:242-7)
  • hypothermia
  • no vaccine in N. America
feline herpesvirus
feline herpesvirus
  • feline rhinotracheitis
  • wide spread in catteries
  • respiratory infections
  • abortions - no direct viral involvement
  • vaccines
marek s disease
Marek’s disease
  • lymphoproliferative, neurological disease of young chickens
  • unilateral paralysis, ataxia due to infiltration of spinal nerves
  • involvement of iris, skin
  • stable in feather follicle dander
beta herpesvirus porcine cytomegalovirus
beta herpesvirus - porcine cytomegalovirus
  • asymptomatic if endemic in herds
  • in susceptible herds
    • inclusion body rhinitis
    • fetal death
    • runting, poor growth in young piglets
malignant catarrhal fever gamma herpesvirus
malignant catarrhal fever (gamma herpesvirus)

other ruminants ?

other species

malignant catarrhal fever

clinical signs in susceptible species
clinical signs in susceptible species
  • peracute
    • high fever, diarrhoea, death in 1-3 days
  • acute
    • fever, depression, enlarged lymph nodes, serous nasal discharge, erosive lesions, corneal opacity, high mortality
  • chronic form
ad