Endocrine Disrupting Chemicals in West Virginia s Waterways: Seasonal Comparisons for Agricultural, Industrial and Resi

1. Endocrine Disrupting Chemicals in West Virginia�s Waterways: Seasonal Comparisons for Agricultural, Industrial and Residential Areas John Cicmanec, EPA, DeeAnn Staats & Paul Gebhard, WV Dept. Environmental Protection, Yongtao Li & J.E. George, Environmental Health Labs, JoBe Hope and Greg Tieman, Triad Engineering

2. Definition of an EDCAn exogenous agent that affects Production Release Transport Metabolism Binding Action Elimination Controlling Homeostasis Development Reproduction

4. Classes of EDCs Fungicides Herbicides Fire Retardants Insecticides Pharmaceuticals Plasticizers Surfactants Effluents Vinclozolin Atrazine PBB ethers Methoxychlor Ethinyl Estradiol Phthalates Alkylphenols Human Sewage

5. Human Exposure Estimates vs Measured Lab Animal Tissue Concentrations

7. Potential Endocrine Disrupting Chemicals Trenbolone acetate (RevalorR, FiniplexR) Melengestrol acetate Estradiol benzoate Ractopamine (PayleanR) Testosterone proprionate Human chorionic gonadotropin Prostaglandins

8. River Water Levels onSampling Dates Potomac R. May 22 July 10 Oct. 21 11,500 cfs 1580 3930 3,200 ave. 1790 2060 Kanawha R. 12500 14900 7500 8300ave. 5600 6500

9. River Water Levels on Sampling Dates (continued) Ohio River May 22 July 10 Oct. 21 72000cfs 38800 57450 30000ave. 25000 22000 Monongahela R. 5200 1600 1430 2000 ave. 450 800 800

10. River Sampling Sites Ohio River Weirton Wheeling Follansbee Sistersville Huntington Monongahela River Morgantown Potomac River Paw Paw Fallingwater Shepherdstown Kanawha River Cedar Grove Montgomery Mt. Carbon Belle St. Albans

11. Ohio River near Sistersville, WV

12. Bellesville Lock & Dam (Ohio River)

13. Kanawaha River at St. Albans, WV

14. Potomac River, Shepherdstown,WV

15. Reproductive Steroid Compounds Compound Estrone Estriol 17 �-Estradiol 17 a-Estradiol 17 a-Ethinyl Estradiol Diethylstilbestrol cis-Testosterone trans-Testosterone Progesterone Minimum Detection Level 0.5 ng/L 0.5 ng/L 0.5 ng/L 0.5 ng/L 0.5 ng/L 0.5 ng/L 0.5 ng/L 0.5 ng/L

16. Alkylphenolic Compounds Compound Nonylphenol, isomer mix Nonylphenol Monoethoxylate Nonylphenol Diethoxylate Bisphenol A Minimum Detection Limit 0.5 �g/L �g/L 1.0 �g/L 0.1 �g/L

17. Rohm-Pelonc Plant St. Albans,WV

18. Farmland near Shepherdstown,WV

19. Summary of Results for Alkylphenolic Compounds Nonylphenol, isomer mix � ranged from 0.6 to 12.0 �g/L with highest level at an agricultural site Nonylphenol Diethoxylate � mostly non-detects but 14 and 17 �g/L detected at two industrial sites in July Bisphenol A was detected at one residential and 3 industrial sites; range was 0.1- 41 �g/L

20. Results of Steroid Hormone Analysis Of 9 compounds being tested, only cis-Testosterone was detected Only five of 156 analyses (3%) were positive The positive results were from industrial/residential locations The positive results occurred at the lowest water levels

21. Results of DEHP Analysis Low levels (0.5 to 2.2 ug/L) were detected frequently The highest level, 6 �g/L, was detected at Follansbee located 600 yards downstream of a WWTP

22. Some Comparisons to the USGS Study, 1999-2000 Frequency of Occurring & Level US Geologic Survey DEHP 11%, 7 �g/L 4-NP 50%, 0.8�g/L Bisphenol A 41%, 0.14�g/L Testosterone 3%, 0.12 ng/L West Virginia DEHP 95%, 1.1 �g/L 4-NP 40%, 2.5�g/L Bisphenol 10%, 5 �g/L Testosterone 8%, 0.9 ng/L

24. Summary Our results indicate moderate incidences and low concentrations for the EDCs that we tested for We would judge that significant dilution occurs in major waterways The presence of specific sources such as WWTP may be significant