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Issues in Biotechnology: The Way We Work With Life Dr. Albert P. Kausch. life edu.org. OnCampus Live BCH 190, MIC 190, AFS 190, NRS 190, PLS 190 OnLine BCH 190. A Sweeping General Survey on Life and Biotechnology A Public Access College Course The University of Rhode Island.

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slide1

Issues in Biotechnology:

The Way We Work With Life

Dr. Albert P. Kausch

lifeedu.org

OnCampus Live

BCH 190, MIC 190, AFS 190, NRS 190, PLS 190

OnLine BCH 190

A Sweeping General Survey on Life and Biotechnology

A Public Access College Course

The University of Rhode Island

Issues in Biotechnology:

Biotechnology, Our Society and Our Future

slide2

Issues in Biotechnology:

The Way We Work With Life

Dr. Albert Kausch

Kimberly Nelson

BCH 190

Section I. The Mechanics of DNA: What is Life?

Section II. The Applications of Biotechnology

A Sweeping General Survey on Life and Biotechnology

A Public Access College Course

The University of Rhode Island

lifeedu.org

slide3

Issues in Biotechnology:

The Way We Work With Life

Dr. Albert P. Kausch

lifeedu.org

The Mechanics of DNA: What is Life?

3.Atoms, Cells and Flow of Life

4. Some Techniques in Biotechnology

A Sweeping General Survey on Life and Biotechnology

The University of Rhode Island

The Flow of Life & The Techniques in Biotechnology

Lectures 3&4

slide4

Issues in Biotechnology:

The Way We Work With Life

Dr. Albert P. Kausch

lifeedu.org

The Mechanics of DNA: What is Life?

4. Some Techniques in Biotechnology

A Sweeping General Survey on Life and Biotechnology

The University of Rhode Island

The Techniques in Biotechnology

Lecture 4

slide5

Proteins Are Used to Copy DNA

DNA does not replicate spontaneously, but is facilitated by a group of proteins.

Interestingly, each of these proteins is coded for in DNA they also replicate.

slide6

Enzymes were discovered that cut DNA

at specific sequences

And subsequently, enzymes were discovered

that paste DNA together.

The ability to cut and paste DNA

allowed gene cloning.

slide7

Plasmids are circular pieces

of DNA found in some bacteria

  • Many copies per cell
  • Antibiotic resistance gene
  • Plasmids can be cut and pasted back together
  • Foreign genes can be inserted
slide8

How is a gene cloned?

Foreign DNA (gene)

is inserted into a plasmid

that has a gene for

antibiotic resistance

The plasmid is introduced into

a bacterial cell and grown on

the antibiotic

Only bacteria with the plasmid

grow…the inserted gene is

copied many times.

slide9

Gene Construction

Promoter

Coding Sequence

Terminator

Protein coding sequence

Cell specificity

Developmental specificity

Start transcription

Stop transcription

Message stability

Gene constructs can be moved into plants and the gene is expressed

driven by the promoter sequence

slide11

Tools and Techniques

used in Biotechnology

slide12

The ultracentrifuge is a centrifuge optimized for spinning a rotor at very high speeds, capable of generating acceleration as high as 2,000,000 G (approx 19 600 km/s²). Ultracentrifuges find important uses in molecular biology, biochemistry and polymer science, including separation of cellular structures and molecules

slide13

Tools of the Trade

The eppendorf tube

and the pipetman

are the standard stock

and trade in the daily

work of a molecular

biologist

slide14

Innovative technologies

become biotech products

“Eppendorf tubes

And Pipetteman

For the Gold Rush”

slide15

A child came to me and said ‘What is the grass?’

fetching it to me

with full hands;

how could I answer the child?

I do not know what it is any more than he.

Whitman

slide16

20. A Pipetman is:

(A) the new biomedical device made by tissue engineering and now used to treat the damaged blood vessels of heart attack victims

(B) a radical group of bioengineered superheroes in the Hollywood movie GATTACCA

(C) a molecular biology tool used in the lab to measure small volumes of liquid common in biotechnology

(D) a new type of bio-engineered crop plants that are drought tolerant

(E) a new surgical tool used in to extract cancer cells

slide17

 21. An ultracentrifuge consists of a rotor that spins tubes containing materials and is:

(A) a component on a new type of microscope to allow cell components to be easily visualized

(B) the laboratory tool developed by Robert Hooke in the 1660s that he used to discover cells

(C) a tool used by cell biologists for separating and comparing cell components based on size and density

(D) a component on the recent Rover Mars mission used to look for life on other planets

(E) used only to enrich uranium for nuclear warheads

slide18

 22. Cloning a gene

(A) is a method that has been banned in Europe but widely used in the US

(B) is a method used to copy a specific segment of DNA

(C) is a method used to reproduce an entire new organism from a donor cell, like

(D) is a science fiction idea that has not yet been accomplished or proven

(E) is a good idea in theory, but has not yet been accomplished

slide19

 23. The atomic number of carbon is 6. Its nucleus must contain:

(A) 6 neutrons and 6 protons.

(B) 3 protons and 3 neutrons.

(C) 6 neutrons and no electrons.

(D) 6 protons and no electrons.

(E) 6 protons and 6 electrons

slide20

 24. A covalent bond is formed when:

(A) two non-polar molecules associate with each other in a

polar environment.

(B) a positively charged particle is attracted to a negatively

charged particle.

(C) one atom gives up electrons to another atom.

(D) two atoms share electrons.

(E) two polar molecules associate with each other in a non-

polar environment.

slide21

25. Enzymes are usually:

(A) proteins

(B) carbohydrates

(C) helpful bacteria

(D) Only available in health food stores

(E) none of these answers are correct

slide22

26. Changing one amino acid within a protein could change what about that protein?

(A) the primary structure of the protein

(B) the overall shape of the protein

(C) the function of the protein itself

(D) the sequence of amino acids specified in the DNA sequence called a gene

(E) all of the above

slide23

27. A shortage of phosphorus would make it difficult for an organism to manufacture

(A) DNA

(B) proteins

(C) cellulose

(D) fats

(E) none of these answers are correct

slide24

 28. Which of the following is not a chemical reaction?

(A) Sugar and oxygen combine to form carbon dioxide and water

(B) sodium metal and chlorine gas unite to form sodium chloride

(C) hydrogen gas combines with oxygen to form water

(D) Ice melts to form liquid water

(E) none of these answers are correct

slide25

29. The chemical units of information in DNA are:

(A) ATGC

(B) UAGC

(C) DAMN

(D) RNAI

(E) XYZQ

slide26

30. In DNA replication an (A) adenine always pairs with

(A) cytosine (C)

(B) thymidine (T)

(C) guanine (G)

(D) tyrosine

(E) monosodium glutamate

slide27

31. Observation of and wonder at the workings of nature are what initiate “why” and “how” type questions. Science is a system of:

(A) relying on one’s best intuition, inspiration and perspiration to solve problems

(B) advertising as a devious enterprise conceived by entrepreneurial western capitalists to make money from the ideas of inventive and often eccentric minds

(C) making theories that fit certain beliefs about why and how things happen

(D) hypothesis making about the mysteries of life

(E) hypothesis making and testing to discern and validate observable facts

slide28

32. Water can absorb and store a large amount of heat while increasing only a few degrees in temperature. Why?

(A) The heat must first be used to break the hydrogen bonds rather than raise the temperature.

(B) The heat must first be used to break the ionic bonds rather than raise the temperature.

(C) The heat must first be used to break the covalent bonds rather than raise the temperature.

(D) An increase in temperature causes an increase in adhesion of the water.

(E) An increase in temperature causes an increase in cohesion of the water.

slide29

33. Water is split during photosynthesis is plants to yield what to compounds?

(A) methane gas and carbon dioxide

(B) oxygen and water

(C) hydrogen and oxygen.

(D) sugars and proteins

(E) RNA and DNA