1 / 9

90 likes | 260 Views

GROWTH EQUATIONS. Biot 309 Fall, 2012. Generation Time. N = (log 10 N f – log 10 N o ) / .301 N Number of generations N f Final Concentration of Cells N o Original concentration of cells .301 Conversion Factor to Convert Log 2 to Log 10. generation (doubling) time.

Download Presentation
## GROWTH EQUATIONS

**An Image/Link below is provided (as is) to download presentation**
Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.
Content is provided to you AS IS for your information and personal use only.
Download presentation by click this link.
While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.
During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

**GROWTH EQUATIONS**Biot 309 Fall, 2012**Generation Time**N = (log10Nf – log10No ) / .301 N Number of generations Nf Final Concentration of Cells No Original concentration of cells .301 Conversion Factor to Convert Log2 to Log10**What is a Logarithm?**• Logarithm is a functionthat gives the exponent in the equation bn = x. It is usually written as logbx = n. • For example: 34 = 81 Therefore log3 81 = 4**Example 1N = (log10Nf – log10No ) / .301**Measure Culture at 9:00 a.m. 10,000 cells / ml Measure Culture at 3:00 p.m. 100,000 cells / ml Calculate N N = (log10Nf – log10No ) / .301 N = (5-4)/0.301 N = 1/0.301 N = 3.33 Generations in 6 Hours We Know: 6 Hours = 360 Minutes Therefore: Generation Time = 360 Minutes / 3.33 Generations N = 108 Minutes to Generate**Example 2N = (log10Nf – log10No ) / .301**Measure Culture at 9:00 a.m. 10,000 cells / ml Measure Culture at Noon 1,000,000 cells / ml Calculate N**Example 2N = (log10Nf – log10No ) / .301**Measure Culture at 9:00 a.m. 10,000 cells / ml Measure Culture at Noon 1,000,000 cells / ml Calculate N N = (log10Nf – log10No ) / .301 N = (6-4)/0.301 N = 2/0.301 N = 6.64 Generations in 3 Hours We Know: 3 Hours = 180 Minutes Therefore: Generation Time = 180 Minutes / 6.64 Generations N = 27 Minutes to Generate**Example 3N = (log10Nf – log10No ) / .301**Measure Culture at 9:00 a.m. 2000 cells / ml Measure Culture at 1:00 p.m. 18,000 cells / ml Calculate N**Example 3N = (log10Nf – log10No ) / .301**Measure Culture at 9:00 a.m. 2000 cells / ml Measure Culture at 1:00 p.m. 18,000 cells / ml Calculate N N = (log10Nf – log10No ) / .301 N = (4.25-3.30)/0.301 N = .95/0.301 N = 3.16 Generations in 4 Hours We Know: 4 Hours = 240 Minutes Therefore: Generation Time = 240 Minutes / 3.16 Generations N = 75.9 Minutes to Generate N = 1.27 Hours to Generate

More Related