1 / 50

Estrous synchronization & ovulation induction

Estrous synchronization & ovulation induction. ชัยวัฒน์ จรัสแสง ภาควิชาศัลยศาสตร์และวิทยาการสืบพันธุ์. References. Hafez, E.S.E. and Hafez, B. Reproduction in farm animals seven edition Gasser, C.L. and Day, M. L. Current and Future Estrous Synchronization Systems .

raphael
Download Presentation

Estrous synchronization & ovulation induction

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Estrous synchronization& ovulation induction ชัยวัฒน์ จรัสแสง ภาควิชาศัลยศาสตร์และวิทยาการสืบพันธุ์

  2. References • Hafez, E.S.E. and Hafez, B. Reproduction in farm animals seven edition • Gasser, C.L. and Day, M. L. Current and Future Estrous Synchronization Systems. • WAAESDHandbook of estrous synchronization

  3. Estrous synchronization • Estrous cycle21 days (17-24 days) • Synchronize or induce • Normal estrous cycle

  4. Synchronization in Farm animals • Days to first breeding (DFB) • voluntary waiting period (VWP) • TAI or Fixed Time AI

  5. Synchronization • Shortening the luteal phase • Luteolysis • PGF2 • Estrogen • Extending the luteal phase • Long term of progestogen 14-21 days • Negative feedback on LH

  6. Advantages of synchronized ovulation • Can synchronize large group to ovulate at same time • Shorten calving season • Reduce labor required for AI breeding • Reduces overall management problems with AI • Market uniform calf crop (same age) • Improves management practices (cattle grouped-closer observation, better feeding practices, etc.)

  7. Disadvantages of synchronized ovulation • Low conception rates • Cost • Concentrated labor required during breeding and calving season • Cows all calving at once (especially bad if have many calving problems)

  8. Compounds used in ovulation synchronization • Progestogens or progestagens 1. Progesterone • Neutralizes the corpus luteum and its natural production of progesterone to rearrange cycle • Used in cattle • CIDR, PRID

  9. Progestogens 2. Synthetic progestogens - Norgestomet Crestar® (implant) - Medroxyacetate progesterone (MAP) Inject - Melengestrol acetate (MGA) (oral)

  10. Prostaglandins • Prostaglandins - Ceases function of the corpus luteum - Must have functional corpus luteum - No effect on developing CL before 5 days of age - No effect on waning CL after about 16 days of age

  11. PGF2 • Natural PGF2 • Dinoprost tromethamine (Lutalyse®) • Synthetic analogue • Cloprostenol sodium Estrumate® • Luprostinal Prosolvin® • Etiproston tromethamine Prostavet®

  12. PGF2 • Prostaglandin analogue a. Very similar to prostaglandin compounds b. Can separate the effects of the prostaglandins (e.g. analogues produce less sweating in mares than prostaglandins)

  13. PGF2 • Intramuscular injection requires higher dosage than intrauterine injection because of the short half-life of the substance a. Metabolizes quickly once in circulation b. Half-life about 2-3 minutes; approximately 90% deactivated in single passage through body Used in cows, ewes, sows and mares IVSM (intravulvosubmucosal) Warning::::::::>>> Causes of abortion

  14. GnRH agonists • Hypothalamus hormone • Release of FSH, LH • Buselerin (Cystorelin®, Factrel®, Fertagyl®, OvaCyst®)

  15. Estrogens • Estradiol conjugates • Estradiol valerate • Estradiol bazoate • Estradiol cypionate

  16. Gonadotropins • eCG or PMSG (equine chorionic gonadotropin or pregnant mare serum gonadotropin) • FSH ( follicle stimulating hormone) • hCG(human chorionic gonadotropin)

  17. Swine synchronization 1. Progestogen (Altrenogest) (Regumate®) • Fed 14-18 days • Normal estrus begins on 4-5 days after withdrawal • Estrus 2-4 days • Fixed-time 6-7 days after progestogen withdrawal Side effects in swine - Development of cystic follicles - Lowered fertility

  18. Swine synchronization 2. Inject 500-1000 IU of hCG • On days 12 • Then Inject PGF2about 3 weeks later

  19. Horses synchronization • Altrenogest (Regumate®) • Fed 15 days • Estrus 3 days after withdrawal • PGF2 • Diestrous • Single injection  estrous 3-5 days • hCG and PGF2 PGF2 hCG PGF2 hCG estrus 1 7-8 15 21-22 2-4 days

  20. Sheep and Goats Synchronization • Ewes estrus (16 days) Does estrus (21 days) • Progestogen (implant or pessary) • Implant 12-14 days sheep • Implant 18-21 days goats • Inject eCG 400-800 IU at withdrawal • TAI ewe at 48-60 hrs and does 30- 48 hrs after progestogen withdrawal

  21. Sheep and Goats Synchronization • PGF2 • Ewes 2 injection 9 days apart double AI at 2-3 days • Does 2 injection 11-12 days apart AI or natural mating at 2-3 days

  22. Cattle synchronization • PGF2 • Two PGF2 11-14 days apart • Estrus 72-96 hrs

  23. Cattle synchronization • Progestogen and PGF2 • Vaginal pessary CIDR or PRID 7 days • PGF2inject at 6 days • AI at Estrus (48-72 hrs)

  24. Cattle synchronization • MGA and PGF2

  25. Cattle synchronization • Progestogen and estrogen • 3 mg Norgestomet implant • 5 mg estradiol valerate inject • removal implant 9 days • AI when esturs or TAI 54 hrs after removal

  26. GnRH and PGF2 • Novel protocols • GnRH agonist + • PGF2 • CIDR • MGA

  27. Heat-Synch ECP at 48hrs

  28. ผสมเทียม เก็บเลือด ตรวจสัด ตรวจสัด ตรวจสัด 45+-3<---14----><---12----><---7---><-46-48ชม.-><--16-20ชม.-> พีจี เก็บเลือด พีจี พีจี จีเอ็นอาร์เอช จีเอ็นอาร์เอช

More Related