HAEMOGLOBINOPATHY CASES on HPLC. Dr. Archana Vazifdar Head Pathologist Hindlabs , HLL Lifecare Ltd. Detector. Direction of flow. HPLC Automated system precalibrated column and gradient. Hemoglobin is eluted in a stepped manner by Buffers of Increasing Ionic strength .
Hindlabs, HLL Lifecare Ltd.
Direction of flow
Automated system precalibrated column and gradient
Hemoglobin is eluted in a stepped manner by Buffers of Increasing Ionic strength
Total Area Count: 1-3 million
HbF: 1-2% RT: 1.03-1.13
HbA2: 1.75-3.25% (2-3.6%) RT: 3.63-3.64
P3- 6% acceptable, 6-15% deterioration
Heterozygous Beta thalassemia
HbA2 LOW in α thalassaemia
All criteria fit provided there is NO history of recent blood transfusion
2 yr/M, anemia, hepatosplenomegaly
28/F, Severe anemia