1 / 29

HAEMOGLOBINOPATHY CASES on HPLC

HAEMOGLOBINOPATHY CASES on HPLC. Dr. Archana Vazifdar Head Pathologist Hindlabs , HLL Lifecare Ltd. Detector. Direction of flow. HPLC Automated system precalibrated column and gradient. Hemoglobin is eluted in a stepped manner by Buffers of Increasing Ionic strength .

ranit
Download Presentation

HAEMOGLOBINOPATHY CASES on HPLC

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. HAEMOGLOBINOPATHY CASESon HPLC Dr. ArchanaVazifdar Head Pathologist Hindlabs, HLL Lifecare Ltd.

  2. Detector Direction of flow HPLC Automated system precalibrated column and gradient

  3. Hemoglobin is eluted in a stepped manner by Buffers of Increasing Ionic strength

  4. Output Time CHROMATOGRAMS Peak Area RT (min) Total Area Count: 1-3 million HbF: 1-2% RT: 1.03-1.13 HbA2: 1.75-3.25% (2-3.6%) RT: 3.63-3.64

  5. P2- Glycemic status, upto 6% acceptable P3- 6% acceptable, 6-15% deterioration 15-25% HbJ

  6. 8 mnth/M, pallor, failure to thrive

  7. Homozygous beta thalassemia

  8. 22/Female

  9. Mother of 8 mnth old child Heterozygous Beta thalassemia

  10. HbA2- Normal • RBC indices- Normal • Silent β thalassaemia • RBC indices s/o thal • Co existing IDA • Co inheritance of α thalassaemia • δβ thalassaemia HbA2 LOW in α thalassaemia

  11. HbLepore Trait

  12. 34/M, Kolkata

  13. Heterozygous E thalassemia

  14. All criteria fit provided there is NO history of recent blood transfusion • ALWAYS CORRELATE with clinical history with CBC & peripheral blood picture • Degenerated sample

  15. Case 1: 2 yr/M, anemia, hepatosplenomegaly Hb 7 MCV 84 MCH 28 MCHC 32.4 RDW 20.2

  16. Elevated HbF: • HPFH (HbF 5-30%, CBC N, asymp) • Heterozygous δβthal • (HbF 3-20%, asymp) • Homozygous beta thal • Pregnancy

  17. Recently transfused c/o homozygous beta thalassemia

  18. Case 2: 28/F, Severe anemia

  19. Borderline HbA2 levels: • Silent carrier of thalassemia • βthal with superadded IDA • Macrocytosis • αβthalassemia

  20. Borderline elevated HbA2 due to macrocytosis

  21. P3: 6% acceptable, 6-12% deterioration 15-25% HbJ

  22. Hb: 6.5 MCV: 63 MCH: 23 MCHC: 26 RDW: 19.6

  23. Double heterozygous for HbE and β thalassaemia

  24. Tests may not be accurate if… • Patient had a blood transfusion within the past four months. • Patient has polycythemia (increased red blood cell production) or underlying anemia • If the patient is on certain medications • Aged/ degenerated sample

More Related