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Vector NTI ( Invitrogen )

Vector NTI ( Invitrogen ). 源資國際生物科技公司 業務專員 陳良坤. Vector NTI Suite.  Vector NTI Nucleotide analyses Restriction site analysis Design Primers  Align  Assemble. Create a new folder. Create a new sequence. View a sequence. Main Menu. Main Toolbar. Pane Selection Toolbar.

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Vector NTI ( Invitrogen )

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  1. Vector NTI (Invitrogen) 源資國際生物科技公司 業務專員 陳良坤

  2. Vector NTI Suite • Vector NTI • Nucleotide analyses • Restriction site analysis • Design Primers •  Align •  Assemble

  3. Create a new folder

  4. Create a new sequence

  5. View a sequence

  6. Main Menu Main Toolbar Pane Selection Toolbar Pane Toolbar Text Pane Graphics Pane Sequence Pane

  7. Download data from NCBI GenBank/GenPetp

  8. File

  9. Reverse sequenece Select all sequence →Right-click mouse →Reverse selection

  10. Find open reading frame (ORF) Analyses →ORF

  11. Translation Select ORF →Analyses →Into New Protein →Direct Strand

  12. Restriction site Analyses →Analyses →Restriction Analyses →Restriction Sites

  13. Cloning by PstI

  14. Design Primers Select region →Analyses →Primer Design →Find PCR Primers

  15. Dimmer and Hairpin of Primers Select primer →Right-click mouse → Analyses

  16. Alignment Select file Align →AlignX-Align Selected Molecules

  17. Assemble Assemble →ContigExpress-Assemble Selected Sequence

  18. Assemble with ABI files Open ContigExpress Project →Project →Add Fragments →From ABI file (no Chinese)

  19. Export data → Right-click mouse →Camera →Select sequence → Right-click mouse →Copy sequence…..

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