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June 12, 2012 | State University of NY. Paul W. Tranchell CIH, CSP, RBP Project Manager. Are there Microbes Lurking?. June 12, 2012 | State University of NY. Are there Microbes Lurking?. Industrial Hygiene. Occupational Stressors: (i.e. biological agents). Anticipation Recognition

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are there microbes lurking
June 12, 2012 | State University of NY

Paul W. Tranchell CIH, CSP, RBP

Project Manager

Are there Microbes Lurking?
agenda
Agenda

Definition

Normal Locations

Selected Suppliers

Unusual & Ancillary Biohazards

Biosafety Fundamentals

biosafety
Biosafety
  • The application of knowledge, techniques and equipment to prevent personal, laboratory, and environmental exposure to potentially infectious agents or biohazards
  • Biosafety defines the containment conditions under which infectious agents can be safely manipulated
  • The objective of containment is to confine biohazards and to reduce the potential exposure of the laboratory worker, persons outside of the laboratory, and the environment to potentially infectious agents
  • Medicine.net http://www.medterms.com/script/main/art.asp?articlekey=33817
normal locations
Normal Locations
  • Bloodborne Pathogens
  • Microbiological Laboratories
  • Animal Laboratories
  • Molecular Biology Labs
select suppliers
Select Suppliers
  • American Type Culture Collection (ATCC) - Repository of a huge number of cell lines
  • CABRI (Common Access to Biological Resources and Information)
  • Developmental Studies Hybridoma Bank - Monoclonal antibodies
  • DSMZ - The DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH = German Collection of Microorganisms and Cell Cultures) is a non-profit public domain general repository specializing in tumor cell lines. It holds the largest collection of human hematopoietic cell lines currently available worldwide.
  • European Collection of Cell Cultures (ECACC) - ECACC "was established in 1984 as a cell culture collection to service the research community and provide an International Depository Authority recognised patent depository for Europe."
  • HyperCLDB - Hypertext resource derived from the Cell Line Data Base (CLDB) with descriptions of more than 4,000 cell lines
  • National Cell Culture Center - A "national resource facility established by the National Institutes of Health (NIH) to provide customized, large scale, cell culture services for basic research laboratories."
unusual ancillary hazards
Unusual & Ancillary Hazards
  • Field Work
    • Lyme Disease
    • Insect Bites
    • Suborder Surpentes
    • Rodent Diseases
      • Plague
      • Hanta Virus
  • HVAC / Legionella
  • Bird Droppings (no bleach please)
  • Mold
  • Suspicious Packages / ”white” powder
  • Drum Skins
chain of infection
Biosafety FundamentalsChain of Infection

Risk Assessment

Reservoir of pathogen

Portal

of

escape

Transmission

Route of entry / infectious dose

Susceptible host

Incubation period

Facilities

Practices / Equipment

Access control / Immunization

PPE

Surveillance

definitions
Biosafety FundamentalsDefinitions

kcd11/HE.prn/NYCER-Biosafety/NYCER_BSL2&3-121311.pptx

control strategies
Biosafety FundamentalsControl Strategies
  • Standard Microbiological Practices
  • Special Microbiological Practices
  • Safety Equipment
    • Primary Barrier
      • Biological Safety Cabinets (BSC)
      • Types, Selection, Use!
    • Personal Protective Equipment
  • Laboratory Facilities
    • Secondary Barriers

kcd11/HE.prn/NYCER-Biosafety/NYCER_BSL2&3-121311.pptx

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