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PLDz2's role in suppressing BFA's inhibitory effects on seedling growth, illustrated through hypocotyl elongation measurements. PLDz2-overexpressing plants exhibited reduced BFA inhibition compared to controls. Statistical analysis supported these findings. Seedlings (n>30) of control, pldz2, and PLDz2-overexpressing plants (LO11, LO19) were grown on media with or without BFA (10 mM) for 7 days under darkness. Bar=2 mm. Error bars show SE, and statistical significance was determined by a one-tailed Student’s t-test (*, P<0.01; #, P<0.05).
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Li and Xue, Supplemental Figure 5 BFA 0 10 µM 25 Control pldz2 20 pO-PLDz2-LO11 LO11 15 LO19 pO-PLDz2-LO19 Hypocotyl length (mm) * 10 # 5 0 BFA 0 10 µM LO11 LO19 C pldz2 C pldz2 pO-PLDz2 pO-PLDz2 Supplemental Figure 5. PLDz2 Suppresses the Inhibitory Effects of BFA on Seedling Growth. BFA inhibition of hypocotyl elongation was suppressed under enhanced expression of PLDz2 (left panel), supported by statistical analysis (right panel). Seedlings (n>30) of WT control, pldz2 and PLDz2-overexpressing plants (LO11, LO19) were grown on media in presence or absence of BFA (10 mM) for 7 days under darkness. Bar=2 mm. Error bars represent SE and a one-tailed Student’s t test indicates significant differences (*, P<0.01; #, P<0.05).