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100bp

a. 500bp. 500bp. 500bp. 100bp. 100bp. 100bp. 1. 1. 1. 2. 2. -4. -. -. -. H2. H2. H2. -. -. -. M2. M2. M2. RAJI. RAJI. RAJI. L540. U937. U937. U937. U698. U698. U698. U266. U266. U266. ladder. ladder. ladder. EHEB. EHEB. EHEB. DG75. ladder. ladder.

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100bp

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  1. a 500bp 500bp 500bp 100bp 100bp 100bp 1 1 1 2 2 -4 - - - H2 H2 H2 - - - M2 M2 M2 RAJI RAJI RAJI L540 U937 U937 U937 U698 U698 U698 U266 U266 U266 ladder ladder ladder EHEB EHEB EHEB DG75 ladder ladder ladder - - - control control control control control control - - - AMO AMO AMO DHL HDLM HDLM BONNA-12 KM KM KM SUP SUP SUP KARPAS-422 - - - SU SU SU 100bp 100bp 100bp 100bp 100bp positive positive positive negative negative negative b Exon Exon 9 9 CTG CTG GTCGTGA GTCGTGA CATGGCGAGC CATGGCGAGC ACCACTCTGC ACCACTCTGC CTGGTTACCC CTGGTTACCC CCCTCACG CCCTCACG TG TG CCCCCCACTG CCCCCCACTG GCCAGGGAAG GCCAGGGAAG CTACCCCACC CTACCCCACC TCCACCCTGG TCCACCCTGG CAGGAATG CAGGAATG GT GT I I ntron ntron 9 9 t t g g a a GCCTG GCCTG g g t t a a g g g g c c a a a a t t g g c c t t g g c c a a g g c c t t g g c c c c t t a a a a t t c c t t a a g g g g t t g g g g g g g g g g g g a a a a c c t t a a a a g g t t c c t t g g a a g g g g c c t t g g g g g g g g t t g g g g g g g g a a a a t t t t g g t t g g g g g g t t g g a a g g c c t t g g c c t t g g a a a a t t g g g g t t c c t t g g t t g g g g a a g g a a c c a a c c a a a a c c g g t t c c c c c c c c t t c c c c c c t t g g c c a a a a a a c c c c a a c c t t g g c c t t a a t t t t c c t t g g t t c c c c c c t t c c t t c c t t Exon Exon 10 10 c c t t c c c c t t t t a a g g AG AG GCTGCAGTTG GCTGCAGTTG GTCCCTCATC GTCCCTCATC CTCCCTCATG CTCCCTCATG AGCAAGCCGG AGCAAGCCGG GGAGGAAGCT GGAGGAAGCT T GCAGAAGT T GCAGAAGT GCCCCCTTGT GCCCCCTTGT GTGCAACCC GTGCAACCC c d 55 kDa 1E+01 1E+00 43 43 kDa 1E-01 PAX2 PAX2 intron 9 / PBGD 1E-02 34 kDa 1E-03 KM-H2 HCT116 1E-04 actin 42 42 kDa kDa 1E-05 lymphoma cell lines solid tumor cell lines colon and melanoma AML /ALL samples blood samples of healthy donors tissue samples

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