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THE EXPERIMENTAL MODELS OF FIRST LIFE OBJECTS. THE ACTIONS OF BIOLOGICAL ACTIVE SUBSTANCES TO THESE MODELS.1Yagolnik E.A., 2Alekseeva O.M., 3Kim Yu. A.1Tula State University, Tula,2N.M.Emmanuel Institute of Biochemical Physics, RAS, Moscow, 3 Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow region E-mails: email@example.com;olgavek@yandex; firstname.lastname@example.org
Model phospholipids membranes imitated the main components of bio-objects. We formed the multilammelar liposomes with aid of hydration of phospholipids thin films under the shacking at warm phosphate buffer.
Biological active substances actions to these experimental objects were tested.
Multilammelar liposomes that were artificially formed from phospholipids bilayer membranes were the adequate model of some intracellular organelles.
Thermo induced endothermic phase
transitions of phospholipids
Pre - transition
Lipid packing from more rankorder to free
Liquid crystal phase
speed of heat supplied (1 degree/min; 0,5 degree/min; 0,25 degree/min; 0,125 degree/min
one-eighth of degree)
to cells with control and experimental samples
5Melafen influence to DMPC thermo inducted parameters – enthalpy, T, cooperatives
ICHFAN C16 formed the its own phase
under the concentrations 10-5, 10-4М
at the belayer. This phase has another
thermo inductions parameters.
The domains of ICHFANs have the great
influencing to the rafts near the integral
and associated receptors and enzymes at
cellular membrane, by this its affect to the
ICHFANs С10 and С12
have the maximal effects, С8 and С16 have
the less effect, probably the length of
anchoring fatty acid residue С10, С12 is
Phenozan has not the fatty
acid tail; by this its influence is weaker.
Phenozan does not formed the own phase.
Its effects were occurred under the
biggest concentrations 10-4, 10-3М
Redundant heat absorbtion
and cell interactions
Next step in bio-structural
complicating may be the
building of hydrophobic peptide
molecules in such membranous
liposomes, or else grabbing
of hydrophilic ones to interior
space. Some regions of big
outer membranes may be
drowning, in interior space,
break away and so that form
small internal liposome with
mixed composition, as the
The education of cell
compartments led on creating
of ionic and electrostatic
gradients that was able to
already emergence of some
Membrane fusion and vesicle introducing, than it were cat and form internal vesicles
Melafen,ICHFAN-10,phenozan influence to the thermo induced protein domains denaturation of erythrocyte ghosts membranes suspension
Erythrocyte ghosts thermograms
А- spektrin, actin; В1-ancyrin, demantin, Band 4,1, 4,2; В2- Band 3 cytoplasm fragment; С- Band 3 – ion channels; D – membrane’s blabbing
2 ICHFAN-10 10-6 М