Major Things Everyone Should Know About Gas Chromatography Column

1. Major Things Everyone Should Know About Gas Chromatography Column The Gas chromatography column is an integral part of the GC apparatus. It is made of glass or stainless steel. Specifically, the glass column is inert and highly fragile. Majorly there are two types of columns namely-Packed columns and capillary columns. In recent times the narrow columns made with fused silica are used extensively, meanwhile on the inner side there is a film of stationary phase. As the resistance to the flow of carrier gas is very low the chromatography columns can be made very long. While designing the high temperatures up to 300 degrees C or more should be taken into account. 

2. We have to select between the Packed columns and the Capillary columns and below is the pointwise major differences which each and everyone should know to make a calculated decision:- The stationary phase which is made of fine particles is fully packed in the Packed columns whereas in Capillary columns on the inner surface of columns the coating of the stationary phase is done.  In Packed columns, the amount of sample required is very high as compared to very less sample required in the case of Capillary columns.  The pressure requirement inside the column is high in the case of Packed columns vis a vis the pressure requirement in the Capillary column.

3. Capillary columns are usually very long as compared to the length of Packed columns. Capillary columns have lengths anywhere between 5 m to 100 m, on the other hand, the length of Packed columns is between 1.5 m to 10 m.  The diameter in the case of the Capillary column is very small around 1 mm and on the contrary, the Packed columns have a diameter up to several millimeters.  We are able to get higher efficiencies when we use Capillary columns and on the opposite, the Packed columns are low performance on the efficiency front. 

4. Packed columns are known for their low resolutions and because of this when we require high resolution the first choice is Capillary columns only.  As the work of Capillary columns is more intricate they are pricey also. The Packed columns are less expensive and are still used at many places due to the economic sense they make.  When it comes to sampling polarity, due to its make of stainless steel the Packed columns are the first choice for working on non-polar samples. Polarity plays a major role in the Capillary columns and further their tubes are made of glass only and are used for separating polar samples. 

5. The requirement of surgical precision in results gave birth to the concept of Zero air. As air does affect very much the sample, the impurities in the air are have had a high tendency to skew with the results. Oxidative catalysis is used to eliminate the hydrocarbons present in the air. Ideally, when the air contains less than 0.1 PPM of total hydrocarbons it can be called zero air. It is advisable to produce zero air in the laboratory itself and for that, you can use Zero Air Generator for Gas Chromatography. For best of the GC columns and Zero air generator please visit https://quadrexcorp.com/ SOURCE http://quadrexcorp.over-blog.com/2021/10/major-things-everyone-should-know-about-gas-chromatography-column.html