Nadia Roan, PhD International AIDS Meeting 2012 July 23, 2012

1. The Effect of Liquefaction on the Ability of Semen and Semen Amyloid Fibrils to Enhance HIV Infection Nadia Roan, PhD International AIDS Meeting 2012 July 23, 2012

2. Title Box

4. Title Box

9. Title Box

11. Title Box

12. Title Box

13. Title Box

15. During semen liquefaction, PSA specifically cleaves SEM proteins

18. Title Box +AEBSF Mock Hours incubation: 0

19. Title Box

21. Summary • Fragments from semenogelins form amyloid fibrils and enhance HIV infection

22. Summary • Fragments from semenogelins form amyloid fibrils and enhance HIV infection • Semen samples naturally defective in semenogelins do not enhance HIV infection

23. Summary • Fragments from semenogelins form amyloid fibrils and enhance HIV infection • Semen samples naturally defective in semenogelins do not enhance HIV infection • Semen that has undergone prolonged liquefaction has diminished ability to enhance HIV infection. The “window of opportunity” for viral enhancement activity is >8 hours post-emission

25. Acknowledgements Gladstone Institute of Virology and Immunology Warner Greene Simon Chu Greene Lab Members University of California, San Francisco Ewa Witkowska Haichuan Liu University of Ulm Frank Kirchhoff Jan Münch Janis Müller A.P. Giannini Medical Research Foundation 1PO1 AI083050-01 PPG Department of Defense #10113005

27. Title Box

28. Title Box

29. Title Box

30. Title Box

31. Title Box

32. J. Müller, J. Münch

33. J. Müller, J. Münch