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Comprehensive Guide to Microbial Sampling Methods

Learn about various approaches, tools, and techniques used to sample and analyze microbial sources for detection, identification, and quantification. This guide covers methods for air, water, surfaces, and more.

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Comprehensive Guide to Microbial Sampling Methods

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  1. Sampling Methods

  2. Sampling • Purpose • Detect and quantify • Identify sources/amplification • Assess potential virulence • Assess effectiveness of controls

  3. Cladosporium

  4. Steps in Sampling • Selection of a laboratory • Selection of analyts • Selection of methods • Sensitivity of method • Field sampling QC • Reporting and lab support

  5. Approaches to Sampling • Qualitative - Type • Quantitative - Concentration

  6. Overview of Microbial Sample Collection Methods(Liquid Impinger)

  7. Overview of Microbial Sample Collection Methods (Fungal Spore Sampling & Spore Traps)

  8. Overview of Microbial Sample Collection Methods(Impactor Samplers)

  9. Overview of Microbial Sample Collection Methods(Sedimentation Settling Plates)

  10. Overview of Microbial Sample Collection Methods(Airborne Particulates & Bulk Samples)

  11. Overview of Microbial Sample Collection Methods(Dust & Tape Lifts)

  12. Tape Lift Sampling

  13. Overview of Microbial SampleCollection Methods(Swab/Wipe & Surface Contact Plate)

  14. Overview of Microbial Sample Collection Methods(Water Sample & Endotoxins in air)

  15. Overview of Microbial Sample Collection Methods(Mycotoxins in Air)

  16. Overview of Microbial Sample Collection Methods (MVOC’s in Air & β-1,3-D-Glucans in Air)

  17. Overview of Microbial Sample Collection Methods(MVOC’s in Air & β-1,3-D-Glucans in Air)

  18. Microbial By Products(Ergosterol in Air) Notes: *Can also analyze for in bulk, surface and water samples. CFU-colony forming units M3-cubic metres of air Mg-milligrams Ng-nanograms Ppm-parts per million Ml-milliliter G-gram **References used to general Table 1 above are listed below 1-8

  19. environmentaleducation.com

  20. Air Flow Valve Biosis Rotameter Pump Plate Sampling

  21. Plate Sampling

  22. Stacked Plate Samplers

  23. Impinger Sampling • Impingement • Liquid media

  24. Impinger Sampling • Types of Impingers • AGI-30 • AGI-4

  25. Impinger Sampling

  26. Air Sampling • Types of Filtration • Membrane filter cassette

  27. Passive Sampling • Deposition • Gravity on agar

  28. Passive Sampling • Types of gravitational • Settling plates

  29. Air Sampling (Spore Trap) • Collects “everything” • Viable,non-viable,pollen, • Unable to differentiate

  30. Carpet Sampling • Collect spores in the carpet pile using air pump • Identifies type of mold • Looking for uncommon types • Provides historical information

  31. Carpet Sampling

  32. Data Interpretation • Concentration inside versus outside. • Mold types inside versus outside. • Presence or absence of a specific mold. • Location of unknown source. • Change in concentration following remediation. • Is a “snap shot” in time.

  33. Stachybotris

  34. Aspergillus

  35. environmentaleducation.com

  36. References

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