Analyses plan module 19
1 / 16

Analyses plan Module 19 - PowerPoint PPT Presentation

  • Uploaded on

Analyses plan Module 19. Major base cations to be determined by ICP-AES Conductivity and temperature {H + } determined using pH electrode Al fractionation Major anions to be determined by IC Use of auto-pipettes Total organic carbon UV and Vis absorption. Conductivity.

I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
Download Presentation

PowerPoint Slideshow about ' Analyses plan Module 19' - preston-bishop

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.

- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
Analyses plan module 19
Analyses planModule 19

  • Major base cations to be determined by ICP-AES

  • Conductivity and temperature

  • {H+} determined using pH electrode

  • Al fractionation

  • Major anions to be determined by IC

    • Use of auto-pipettes

  • Total organic carbon

    • UV and Vis absorption


  • Master student lab V160

  • Ecoscan Con5 (Eutech instruments) conductivity meter.

    • The instrument is calibrated using 1000 and 1433 µS calibration solutions

  • The measurements are done for quality control purposes in order to compare measured and calculated conductivity

H determined using ph electrode
{H+} determined using pH electrode

  • Analytical Chemistry lab Ø109

  • Thermo Orion model 720 pH-meter with a Blueline 11-pH electrode.

    • The pH-meter is calibrated with pH = 4.00 and 7.01 buffer solutions

Major base cations to be determined by icp aes
Major base cations to be determined by ICP-AES

  • Ca2+, Mg2+, Na+, K+

    • Method will be demonstrated in Module 24

      • Appropriate calibration solutions are prepared by Masha

      • Conducted by Anne-Marie Skramstad

Major anions to be determined by ion chromathograph ic
Major anions to be determined by Ion Chromathograph (IC)

  • Analytical Chemistry lab Ø109

  • Tot-F, Cl-, NO3-, SO42-

    • Principle

      • The sample is injected in a flow of eluent

      • The analyte ions are separated by different degree of binding to the active sites on the ion exchange material

      • Ions with opposite charge of the analyte is exchanged with H+ or OH-

      • The activity of the analyte is and accompanied H+ or OH- in the eluent stream is measured by means of a conductometer

    • Presented by Hege Lynne et al

Total organic carbon
Total organic carbon

  • Analytical chemistry lab Ø 104

  • High temperature (680C) catalytic combustion analysis on a Shimadzu TOC-5000A instrument

  • Principle:

    • The organic carbon is combusted to CO2 by high temperature and catalysis. The amount of CO2 produced is measured using av IR detector

  • Presented by Hege Lynne et al.

    • Analytes measured may include: TC, IC, TOC, NPOC, and POC

Al fractionation
Al fractionation

  • Master student lab V160

  • Method presented as example in Lecture 1 (slide 15)

  • Download manual from


Qc of data
QC of data

  • After the analysis the data must be compiled and quality controlled by ion balance and agreement between measured and calculated conductivity

  • For this purpose you may use the Data compilation and QC worksheet available at

Species in natural freshwater central equilibriums in natural water samples

Speciesin natural freshwaterCentral equilibriums in natural water samples

KJM MEF 4010

Module 19

Inorganic complexes
Inorganic complexes

  • Major cations in natural waters

    • H+, Ca2+, Mg2+, Na+, K+

  • Common ligands in natural systems:

    • OH-, HCO3-, CO32-, Cl-, SO42-, F- & organic anions

    • In anoxic environment: HS- & S2-

  • Dominating species in aerobic freshwater at pH 8 are:


  • In aqueous systems, hydrolysis reactions are important

    • Hydrolysis reactions are controlled by {H+}

      • The higher the pH, the stronger the hydrolysis of metal cations

      • E.g. Aluminium

        • Al3+aq denotes Al(H2O)63+

Concentrations of dissolved fe 3 species two total fe concentrations fe t 10 4 m and fe t 10 2 m
Concentrations of dissolved Fe3+ speciesTwo total Fe concentrations, FeT = 10-4M and FeT = 10-2M

Dissolved organic matter
Dissolved Organic Matter

  • Low molecular weight (LMW)

    • < 1000Da (e.g. C32H80O33N5P0.3)

    • E.g.:

  • High molecular weight

    • 1000 - > 100 000Da

    • Humic substance

      • Very complex and coloured substances

  • Enhances weathering

    • The protolyzation of weak organic acids

    • Complexation of Al and Fe

      • Total congruent dissolution


  • {X}=X · [X]

    • {X} is the activity to X

    • [X] is the concentration to X

    • X is the activity coefficient to X

      • X are dimensionless

      • It is determined by:

        • The diameter (å) of the hydrated X

        • Its valence (nX)

        • The ionic strength (I)

Not possible to calculate further than I=0.1





  •   when I  0 1 when I<10-5M

Anions + cations

Debye huckel dh equation
Debye Huckel(DH) equation

  • For ionic strengths (I) < 0.1M the X can be calculated by means of e.g. the Debye Huckel equation:

    I < 0.1

    I < 0.005

    • 0.5 & 0.33 are temperature dependent table values

      • Presented values are for 25°C

    • åX is a table value for the specie in question