1 / 2

六味地黃丸與毛葉腎蕨降血糖活性成分之探討

六味地黃丸與毛葉腎蕨降血糖活性成分之探討.

pravat
Download Presentation

六味地黃丸與毛葉腎蕨降血糖活性成分之探討

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. 六味地黃丸與毛葉腎蕨降血糖活性成分之探討 • 本研究探討六味地黃丸及毛葉腎蕨之活性成分，針對初期篩檢具有降血糖活性的六味地黃丸之正丁醇層，及毛葉腎蕨Nephrolepis multiflora (Roxb.) Jarrett ex Morton的二氯甲烷層，做進一步的活性成分探討；地黃為六味地黃丸中的君藥，而地黃因炮製不同分為生地黃與熟地黃，故以降血糖作用之差異及炮製前後的成分變化作進ㄧ步的探討。經各種層析管柱及不同溶媒系統分離純化後，共分離出二十二個化合物。六味地黃丸於n-BuOH層分離出六個化合物，分別為5-hydroxymethyl-2-furaldehyde (1)、2-hydroxy-5-hydroxymethylfuran (2)、5-[(α-D-Galactopyranosyloxy)methyl]-2-furancarboxaldehyde (3)、loganin (4)、paeoniflorin (5)、oxypaeoniflorin (6)；生地黃於n-BuOH層分離出八個化合物，分別為catalpol (7)、dihydrocatalpol (8)、ajugol (9)、scutelloside (10)、uridine (11)、sucrose (12)、raffinose (13)、stachyose (14)，其中scutelloside則首次在生地黃中分離出來。另外，從毛葉腎蕨共分離八個化合物，其中自二氯甲烷層萃取物得到的分別為arctigenin (15)、arctiin (16)、palmitic acid (17)。自乙酸乙脂層萃取得到ethyl-β-D-fructopyranoside (18)、shikimic acid (19)、ethyl shikimate (20)、matairesinoside (21)、astragalin (22)，而compound 15~16 及19~21為首次在此植物中發現。經初步藥理活性評估，在對C2C12 cell glucose uptake之作用，發現六味地黃丸中的compound 1、4，生地黃中的compound 7、8、13，毛葉腎蕨中的compound 19、20有促進作用。其中除了loganin (4) 及 catalpol (7) 已有文獻發表之外，compound 1、8、13、19、20則為首次發現具有glucose uptake的促進效果。在生地黃中，由於catalpol (7) 及rdihydrocatalpol (8) 相較於熟地黃含量較多，故推測生地黃的降血糖效果優於熟地黃。

  2. Studies on the Hypoglycemic Constituents of Liuwei Dihuang Pills and Nephrolepis multiflora • In this study, bioactive constituents of Liuwei Dihuang Pills and Nephrolepis multiflora (Roxb.) Jarrett ex Morton were investigated. n-BuOH extracts of Liuwei Dihuang Pills and CH2Cl2 extracts of Nephrolepis multiflora (Roxb.) Jarrett ex Morton are obviously hypoglycemic activity. Rehmannia glutinosa Libosch. is the principal herbs (jun yao) of Liuwei Dihuang Pills. When Rehmannia glutinosa Libosch. (di huang) has been just prepared by drying and steaming. They are called sheng di huang (means raw) and shu di huang (means cooked), respectively. Investigating the changes in chemical components of Rehmannia glutinosa Libosch. during processing. Through all kinds of different solvents and chromatography system, a total of 22 compounds isolated. The known compounds which have isolated from the n-BuOH layer of Liuwei Dihuang Pills are 5-hydroxymethyl-2-furaldehyde (1), 2-hydroxy-5-hydroxymethylfuran (2), 5-[(α-D-Galactopyranosyloxy)methyl]-2- furancarboxaldehyde (3), loganin (4), paeoniflorin (5), oxypaeoniflorin (6). The known compounds which have isolated from the n-BuOH layer of Rehmannia glutinosa Libosch. (sheng di huang) are catalpol (7), dihydrocatalpol (8), ajugol (9), scutelloside (10), uridine (11), sucrose (12), raffinose (13) and stachyose (14). Scutelloside (10) is the first report of Rehmannia glutinosa Libosch. As for the compounds which have isolated from the CH2Cl2 layer of Nephrolepis multiflora ( Roxb. ) Jarrett ex Morton are arctigenin (15), arctiin (16) and palmitic acid (17). In addition, the compounds which have isolated from the CH2Cl2 layer of Nephrolepis multiflora ( Roxb. ) Jarrett ex Morton are ethyl-β-D-fructopyranoside (18), shikimic acid (19), ethyl shikimate (20), matairesinoside (21) and astragalin (22). Compound 15~16 and 19~21 are the first report of Nephrolepis multiflora ( Roxb. ) Jarrett ex Morton. Among them, 5-hydroxymethyl-2-furaldehyde (1), loganin (4), catalpol (7), dihydrocatalpol (8), raffinose (13), shikimic acid (19) and ethyl shikimate (20) can enhance glucose uptake in C2C12 experiment. Catalpol (7) is the indicator compound in sheng di huang. The content of dihydrocatalpol (8) in sheng di huang is more than the shu di huang. Therefore, the hypoglycemic activity of sheng di huang is better than the shu di huang.

More Related