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Supported by grant from Brazilian Scientific Foundations: FAPESP (08/53969-0), CAPES, CNPq. - PowerPoint PPT Presentation


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Fabio Carvalho , Linda C Carrijo-Carvalho , Ana Marisa Chudzinski-Tavassi, Annette Foronda, Denise de Freitas . Low-molecular weight proteins as potential virulence factors of Acanthamoeba pathogens in corneal infection.

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Presentation Transcript
slide1
Fabio Carvalho, Linda C Carrijo-Carvalho, Ana Marisa Chudzinski-Tavassi, Annette Foronda, Denise de Freitas

Low-molecular weight proteins as potential virulence factors of Acanthamoeba pathogensin corneal infection

Supported by grant from Brazilian Scientific Foundations: FAPESP (08/53969-0), CAPES, CNPq.

background
Corneal infections due to free-living amoebae have potentially devastating consequences. If not successfully treated, it can progress into the eye, causing corneal ulcer, loss of visual acuity and eventually blindness.BACKGROUND
background1
Proteases represent a class of enzymes which are related with pathogenicity and cytolysis of Acanthamoeba species and genotypes.BACKGROUND
purpose
To evaluate the expression of extracellular proteins by Acanthamoeba trophozoites as predictive virulence factors in the corneal infection by the protozoa. PURPOSE
methods
The research was conducted in accordance with the tenets of the Declaration of Helsinki
  • Corneal scrapings
    • 10 patients
    • Contact-lens wearers
  • Acanthamoeba keratitis isolates (n=10)
METHODS
results
KDa

109

95

46

36

RESULTS

Lane 1:

  • Negative control

Lane 2:

  • ATCC 30011 avirulent strain

Lanes 3 and 6:

  • Single pattern

Lane 4:

  • High diversity
results1
KDa

141

117

96

76

55

36

RESULTS

Lane 8:

  • Single pattern

Lanes 11 and 12:

  • High diversity
slide9
Collagenases
  • AK: role in virulence.

Low molecular weight protein

conclusions
Heterogeneous extracellular collagenases are produced by different Acanthamoeba isolates;
  • Avirulent Acanthamoeba strain could not produce collagenolytic enzymes;
CONCLUSIONS
conclusions1
Collagenases should be suggested as a virulence marker in the differentiation of Acanthamoeba species and genotypes.CONCLUSIONS
perspectives
The genotyping and phylogenetic inferences of clinical isolates are currently under investigation;
  • Collagenolytic enzymes are under isolation by chromatographic techniques.
PERSPECTIVES
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