cell division dynamics a closer look at gastrulation in zebrafish l.
Download
Skip this Video
Loading SlideShow in 5 Seconds..
Cell Division Dynamics A Closer Look at Gastrulation in Zebrafish PowerPoint Presentation
Download Presentation
Cell Division Dynamics A Closer Look at Gastrulation in Zebrafish

Loading in 2 Seconds...

play fullscreen
1 / 26

Cell Division Dynamics A Closer Look at Gastrulation in Zebrafish - PowerPoint PPT Presentation


  • 147 Views
  • Uploaded on

Cell Division Dynamics A Closer Look at Gastrulation in Zebrafish. Salome Chuang, Daniel Holland-Moritz Mentor: Luca Caneparo Summer Research Connection Oak Crest Institute of Science California Institute of Technology July 30, 2010. Cell Division: A Crucial Biological Process. Normal:

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'Cell Division Dynamics A Closer Look at Gastrulation in Zebrafish' - plato


Download Now An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
cell division dynamics a closer look at gastrulation in zebrafish
Cell Division Dynamics A Closer Look at Gastrulation in Zebrafish
  • Salome Chuang, Daniel Holland-Moritz
  • Mentor: Luca Caneparo
  • Summer Research Connection
  • Oak Crest Institute of Science
  • California Institute of Technology
  • July 30, 2010
cell division a crucial biological process
Cell Division: A Crucial Biological Process
  • Normal:
    • Growth
    • Development
    • Tissue repair
  • Abnormal:
    • Cancer

http://tainano.com/chin/Molecular%20Biology%20Glossary.htm

our project dynamics of cell division in embryos

Spindle Poles

Our Project: Dynamics of Cell Division in Embryos

End of Cell Division

Beginning of Cell Division

difficulties of studying cell division in live embryos
Difficulties of Studying Cell Division in Live Embryos
  • Ethical concerns (humans)
  • Opaque embryos
  • Development Rate
  • Production Rate
  • Manipulation
  • Storage/Care
  • External vs. Internal
  • Development

Images from Kidscientist.com and odec.ca

zebrafish an ideal model for studying embryonic cell division
Zebrafish: An Ideal Model for Studying Embryonic Cell Division
  • Very common for use in research because of:
    • Rapid reproductive rate
    • Transparent embryos
    • Rapid development
    • Easily cared for and contained
    • Easy to induce and screen for mutations
    • Genome almost fully sequenced

Bar: 250 µm

Images from focusonnature.be

a brief history on zebrafish
A Brief History on Zebrafish

George Streisinger

Established zebrafish as an ideal subject for imaging and manipulation.

Very small species of minnow

Native to the Ganges region of India

Named for distinctive stripes

Since then zebrafish have been popularized as a model for studying vertebrate development:

Cell biology and development

Organ system development

Genetic studies

Image from www.zfin.org

stages of zebrafish development
Stages of Zebrafish Development

Key:

=Injection stage

=Imaging stage

significance of the dorsal region
Significance of the Dorsal Region

Dorsal region becomes...

Nervous system

Application:

Cell orientation could have an impact on brain and nervous system development at an early age

Cell imaging provides better insight into....

Embryonic development

Cell division mechanisms and signaling in vertebrates

shield stage to gastrulation trends
Shield Stage to Gastrulation: Trends
  • Tissue differentiation
    • Dorsal region: Definite pattern
    • Cells divide along the anterior-posterior plane

Concha, Adams, 1998

visualizing cell division dynamics

Spindle Poles

Visualizing Cell Division Dynamics

End of Cell Division

Beginning of Cell Division

methods genetic engineering of fluorescent embryos
MethodsGenetic Engineering of Fluorescent Embryos
  • mRNA codes for fluorescent protein in cytoskeleton and nucleus

DNA  RNA  Protein

  • Synthetic mRNA produced for injection
  • mRNA injected into embryos at one cell stage

Image from protein-dynamics.l001.de

methods imaging of embryos using confocal microscopy
MethodsImaging of Embryos Using Confocal Microscopy
  • Confocal microscope detects fluorescence
  • Embryos oriented to image dorsal region
  • Anterior and posterior marked
  • Imaged over a period of 4 hours
analysis
Point assigned to each half of separating chromosomes

Program tracks and measures the angle of division relative to the anterior-posterior plane and dorsal-ventral plane

Analysis
  • Special computer program tracks division alignment

Image Courtesy of L. Caneparo

results
Results

We observed an active rotation of the cells to align

prior to division.

Before

After

future directions
Future Directions
  • Further Analysis
    • Quantify angle/character of rotation
    • Statistically analyze data
  • Investigate cell signaling patterns
  • Compare cell division in other vertebrate models
  • Explore implications on neural development
acknowledgements
Acknowledgements
  • Special Thanks to:
  • Luca Caneparo
  • Fraser Lab
  • Sherry Tsai
  • James Maloney
  • California Institute of Technology
  • Howard Hughes Medical Institute
  • Siemens Foundation
  • Our families