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Growth Comparison of Dcys Mutants in M9 Medium with Cystine Supplementation

Study comparing growth of Dcys mutants with cystine supplementation in M9 medium. Includes WT, ΔcysE, ΔcysK, ΔcysM, and double knockout mutants. Cultures streaked on M9 and M9+5mM cystine plates.

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Growth Comparison of Dcys Mutants in M9 Medium with Cystine Supplementation

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  1. Supplemental Figure 3 M9+Cystine （5 mM） M9 Supplemental Figure 3Growth of DcysKDcysM double knockout mutant on minimum medium WT (BW25113), DcysE, DcysK, DcysM and DcysKDcysM were cultivated in M9 medium until OD660=0.4 at 37°C. The cultures were streaked and cultured on M9 and M9+ 5 mMcystine plate at 37°C. M9 and M9+ 5 mMcystine plate contains 1 mM Na2S2O3 and 1 mM MgSO4 as inorganic sulfur sources. WT ΔｃｙｓE ΔｃｙｓK ΔｃｙｓM ΔｃｙｓK ΔｃｙｓM

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