1 / 9

Chapter Six – Separation Methods

Explore the various separation methods utilized in forensic analysis, from liquid and solid phase extractions to chromatography and electrophoresis. Understand the principles and applications of gas and liquid chromatography, along with thin-layer chromatography and electrophoresis. Dive into the history, theory, and components of each method to enhance your forensic analytical skills.

phong
Download Presentation

Chapter Six – Separation Methods

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Chapter Six – Separation Methods FRSC 8104 Criminalistics II Professor Bensley

  2. FRSC 8104 – Chapter 6 Objectives After studying this chapter, the student should be able to:  Describe solid and liquid phase extractions and recognize when these techniques are useful  Understand the basic concepts behind chromatographic methods Recall the important components of a gas chromatograph and a high performance liquid chromatograph and their function  Recognize how chromatographic techniques are employed in the forensic laboratory; know which separation techniques are useful for the various types of evidence encountered in the laboratory

  3. FRSC 8104 – Chapter 6 Outline • Introduction • Why are these techniques needed? • Definitions: Analyte, Solvent, Solute • Liquid Phase Extraction • Partitioning Process • Polarity • pH

  4. FRSC 8104 – Chapter 6 Outline

  5. FRSC 8104 – Chapter 6 Outline • Solid Phase Extraction • Adsorption • Examples

  6. FRSC 8104 – Chapter 6 Outline • Chromatography • History and Theory • Stationary vs. Mobile Phases • Normal and Reverse Phases • Gas Chromatography (GC or GLC) • Packed vs. Capillary Columns • Components • Injection Port • Column • Detector • Quantitative Analysis • Pyrolysis GC • Applications

  7. FRSC 8104 – Chapter 6 Outline • High Performance Liquid Chromatography (HPLC) • Advantages over GC • Components • Injector • Column • Detector • Applications

  8. FRSC 8104 – Chapter 6 Outline • Thin Layer Chromatography (TLC) • Stationary vs. Mobile Phases • Process • Detection • Applications • Advantages/ Disadvantages

  9. FRSC 8104 – Chapter 6 Outline • Electrophoresis • Gel vs. Capillary (CE) • Stationary Phases • Mobile Phase • Detectors • Applications Animation Detailing Capillary Electrophoresis

More Related