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Visualization of Stroma-Targeted DsRed and Chlorophyll Autofluorescence in Living Mesophyll Cells

This study illustrates the use of stroma-targeted DsRed and chlorophyll autofluorescence for visualizing living mesophyll cells from atg4a4b-1 plants. Fresh leaves from control conditions and IDLs were analyzed immediately after a 5-day treatment. The leaves were treated with 1 μM concanamycin A in a buffered solution before observation. Stromal-targeted DsRed emits green fluorescence while chlorophyll shows red. In merged images, the interaction of DsRed and chlorophyll produces yellow fluorescence, facilitating detailed analysis of mesophyll cell structures.

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Visualization of Stroma-Targeted DsRed and Chlorophyll Autofluorescence in Living Mesophyll Cells

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  1. Supplemental Figure S2. Visualization of stroma-targeted DsRed and chlorophyll autofluorescence in living mesophyll cells of atg4a4b-1. A and C, fresh leaves excised from the control condition leaves (A) and IDLs (C) after the 5-d treatment were observed immediately. B and D, excised control leaves (B) and IDLs (D) were incubated with 1 μM concanamycin A in 10 mM MES-NaOH (pH 5.5) at 23C for 20 h in darkness. Stromal-targeted DsRed appears green and chlorophyll fluorescence appears red. In merged images, overlap of DsRed and chlorophyll fluorescence appears yellow. Bars = 25 μm.

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