1 / 15

Igor Sharakhov

Igor Sharakhov. High Rates of Genome Rearrangements in Malaria Mosquitoes, Anopheles gambiae and A. funestus. University of Notre Dame, USA. Genus Anopheles Subgenus Cellia. Series Pyretophorus gambiae species complex. Myzomyia funestus group. A. gambiae. A. funestus.

palmer
Download Presentation

Igor Sharakhov

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Igor Sharakhov High Rates of Genome Rearrangements in Malaria Mosquitoes, Anopheles gambiae and A. funestus University of Notre Dame, USA

  2. GenusAnopheles Subgenus Cellia • Series Pyretophorus • gambiae species complex • Myzomyia • funestus group A. gambiae A. funestus

  3. FISH on chromosomes of A. funestus Of 157 cDNAs used as probes, 116 mapped to single chromosomal locations, and the remainder hybridized in multiple locations

  4. Synteny at the whole arm level • Reciprocal whole arm translocation between 2L and 3R

  5. Gene order comparison • funestus • A. gambiae Length of X – 24.9Mb Mean length of conserved segments - 2.0Mb0.2Mb Number of inversions - 51 (Nadeau &Taylor, PNASUSA. 1984 Feb;81(3):814-8 )

  6. funestus • A. gambiae Mean length of conserved segments - 0.9Mb0.2Mb Number of inversions - 369 • funestus • A. gambiae Mean length of conserved segments - 2.2Mb1.0Mb Number of inversions - 113

  7. funestus • A. gambiae Mean length of conserved segments - 2.2Mb0.4Mb Number of inversions - 113 • funestus • A. gambiae Mean length of conserved segments - 1.1Mb0.4Mb Number of inversions - 195

  8. Rate of gene order rearrangement The divergence time between A. gambiae and A. funestus lineages is ~ 5 My

  9. Attachments of chromosomes to the nuclear envelope (NE) A. funestus A. gambiae (Sharakhov et al. Genetics. 2001 Sep;159(1):211-8)

  10. Function of nuclear organization in interphase chromosome interactions (Marshall WF, Curr Biol. 2002 Mar 5;12(5):R185-92)

  11. Conclusions • Gene order has being shuffled at a high rate • Paracentric inversions have had a major impact on the genome rearrangement • Genomic segments evolve at different rates

  12. University of Notre Dame, USA • Andrew Serazin, Olga Grushko, Ali Dana, Neil Lobo, Maureen Hillenmeyer, Frank Collins, Nora Besansky • Purdue University, USA • Richard Westerman, Jeanne Romero-Severson • Centre National de Recherche et de Formation sur le Paludisme, Burkina Faso • N’Fale Sagnon, Carlo Costantini This work was supported by grants from the National Institutes of Health (AI48842) to N.B. and from the Indiana 21st Century Research & Technology Fund to F.C.

More Related