Vanderbilt University Mass Spectrometry Core Services. David L. Hachey, PhD Director of Mass Spectrometry Professor of Pharmacology, Biochemistry, Chemistry. … An Overview.
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David L. Hachey, PhD
Director of Mass Spectrometry
Professor of Pharmacology, Biochemistry, Chemistry
MS CoreDavid Hachey
Res Assist. Prof
Hewlett-Packard (Agilent) - 5989A
ThermoElectron (Finnigan) - DSQ
ThermoElectron (Finnigan) - TSQ 7000
" - TSQ Quantum (x2)
" - TSQ Quantum Ultra
" - LTQ Linear ion trap
" - LCQ Deca XP
Applied Biosystems - Voyager Elite
" - Voyager STR
Open-access Instrument Lab - 2,000 sq. ft.
Sample Processing Lab - 575 sq. ft.
Data Analysis Workstation Lab - 96 sq. ft.
Standardized Log Abundance
samples are co-resolved
Quantitative inter-comparison of multiple samples (in replicate)
MALDI-TOF MS and TOF/TOF MS/MS on targeted proteins
L to R: Julie Coleman, Michele Reyzer,PhD;, Olivia Faison, Erin Seeley,PhD, and Lisa Zimmerman, PhD.
Direct proteome profiling and protein imaging of intact tissue by MALDI-MS.
Tissue Profiling typically involves many samples of several types, and the goal is often to discover patterns in the protein profiles of the samples that can classify the samples based on biological state. (e.g., tumor vs. normal) and that can predict biological outcomes (e.g., the prognosis of a patient).
Tissue Imagingis usually performed on a small number of samples where the goal is to obtain a relatively high resolution image showing the distribution of various proteins in the tissue section.
Proteome profiling of serum, plasma and other biofluids by MALDI-MS
generates spectral patterns in the protein profiles as a tool used to differentiate between disease states.
Multidimensional Analysis (LC-MS-MS) of Biofluids
comprehensive analysis of fluids providing inventory of protein identified in samples.
MS images of mouse kidneys infected with S. aureus. The signal at m/z 10,165, tentatively identified as calgranulin A, is present in the kidney abscesses, but is absent in healthy tissue. The signal at m/z 12,135 appears down-regulated in infected tissue and is absent in abscessed tissue.