Isoaspartyl Peptidase Activity Assay: Determination of Kinetic Parameters with b-Asp-His Substrate

Feb 27, 2025

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This assay determines isoaspartyl-peptidase activity by measuring Km value using b-Asp-His substrate at 10.5nkat/mg-1. Analyze the enzymatic activity at 0.54mM concentration for research purposes.

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isoaspartyl peptidase
activity assay
kinetic parameters
substrate
enzyme
assay method
protein analysis

otylia

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Isoaspartyl Peptidase Activity Assay: Determination of Kinetic Parameters with b-Asp-His Substrate

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20 15 Isoaspartyl-peptidase Act. (nkat mg-1) Km= 0.54 mM 10 5 0 2 6 8 4 0 [b-Asp-His] mM

M A Y 2 0 0 7

M A Y 2 0 0 7 T E L E C O M S T O C K : R E C O M M E N D A T I O N Presentation to FIN 824 (SPRING QUARTER) Marc Reitter Siddhesh Sankulkar T E L E C O M S T O C K R E C O M M E N D A T I O N Telecom Stock Recommendation Overview: 1

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M A Y 2 0 0 7

M A Y 2 0 0 7. T E L E C O M S E C T O R : O V E R V I E W . Presentation to FIN 824 (SPRING QUARTER) Marc Reitter Siddhesh Sankulkar. T E L E C O M S E C T O R. Telecom Sector Overview: . 1. Sector Overview. 2. 2.

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n=4,m=0

The HSX Experimental Program. F. Simon B. Anderson , D.T. Anderson, A.R . Briesemeister , C. Clark, K.M.Likin , J. Lore, H. Lu, P.H. Probert , J.W. Radder , J. Schmitt , J.N . Talmadge , R. Wilcox, K . Zhai HSX Plasma Laboratory, University of Wisconsin

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Mm, Mm, Mm, Mm is for moon . Mm, Mm, Mm, Mm is for mouse . Mm, / m /, moon .

Let’s Chant!. Mm, Mm, Mm, Mm is for moon . Mm, Mm, Mm, Mm is for mouse . Mm, / m /, moon . Mm, / m /, mouse . Mm, / m /, _____. Let’s find!. much. Mary. me. my. milk. Let’s Read!. m ac, m ad, m an, m ap, m at, m eg , m en , m ess , m et ,

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T M K

T M K. Foods. “Bringing you a taste of America”. Buyers Prospective, Exporting To Europe, Generating Sales. Meeting The Buyer, European Labeling Requirements, Quality Control. Pricing, Sales Cycle, Keeping In Touch, Q&A. Presented by Steve Scott TMK Foods. T M K. Foods.

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K M

E C O N O M I C C O N S U L T A N T S. K M. March 26th, 2002. Compensation Awards and Real Returns. Presentation by Colm Mc Carthy DKM Economic Consultants. 1. Introduction. The Real Rate - 4% or 2.5%? What the Record Shows Index-Linked Yields

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D 0  K + K - Signal

Milano Ntuples , 2952 runs. D 0  K + K - Signal. Sezdee base cuts L/S > 5. L/Sig > 9 K not P > -2 DCL cut > .02 D0_mom > 40 GeV/C^2 dikaon mom. asymm. Cut < .6 -reflection peaks more distinct. Primarily Kpp 0. Primarily Kp. all of the above cuts Min. dw > 3

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Mm, Mm, Mm, Mm is for moon . Mm, Mm, Mm, Mm is for mouse . Mm, / m /, moon .

Let’s Chant!. Mm, Mm, Mm, Mm is for moon . Mm, Mm, Mm, Mm is for mouse . Mm, / m /, moon . Mm, / m /, mouse. Mm, / m /, _____. Let’s find!. much. Mary. me. my. milk. Let’s Read!. m ac, m ad, m an, m ap, m at, m eg , m en , m ess , m et ,

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Treatment Sucrose (mM) Nitrate (mM) 1 30 0 2 30 0 3 60 0 4 60 0 5 90 0 6 90 0

Supplemental Figure 1. Different modes of regulation in response to CN.

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KTeV Results: K L  p 0 ll ( l =e, m , n )

KTeV Results: K L  p 0 ll ( l =e, m , n ). BEACH 2004 June 30, 2004 Julie Whitmore, Fermilab Physics Motivation - Direct CP Violation KTeV Detector Results Summary The KTeV Collaboration: Arizona, Campinas, Chicago, Colorado, Elmhurst, Fermilab, Osaka,

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Measurement of the Amplitude Ratio of B 0  J/  K 0 to B 0  J/  K 0 (K +  - ) decays

Measurement of the Amplitude Ratio of B 0  J/  K *0 to B 0  J/  K *0 (K +  - ) decays. Max Baak NIKHEF, Amsterdam For the BaBar Collaboration APS Meeting Philadelphia, 8 April 2003. Two simple concepts. Wrong-flavor decay. ?. B 0  J/ K * 0 B 0  J/ K * 0. ( ).

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Search for Super-Penguins: CP Violation in B 0 ->K+K-K 0

Search for Super-Penguins: CP Violation in B 0 ->K+K-K 0. SM rules. D. Dujmic, SLAC For BABAR Collaboration. Special thanks to: G. Cavoto, M. Krishnamurthy, E. Di Marco, M.Pierini, A.Roodman, S.Spanier, J.Thompson. p=u,c,t. W ±. V pq. ?. q=d’,s’,b’. CP Violation in SM.

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Analysis of ratio BR( K     0 )/BR(K    )

Analysis of ratio BR( K     0 )/BR(K    ). Motivation Selection and cuts Trigger efficiency Signal and Background fit Selection efficiency Result and conclusion. M. Martemianov V. Kulikov. Motivation / I.

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D 1 -D 2 lifetime difference using D 0 K  K  , D 0      and D 0 K   

D 1 -D 2 lifetime difference using D 0 K  K  , D 0      and D 0 K   . W. Lockman, C. Chavez, J. Coleman, R. Cowan, K. Flood, B. Petersen BaBar Physics Workshop June 30 - July 2, 2008. Introduction Analysis Fit results Cross checks and validations Systematics.

453 views • 34 slides

L. 175,00 mm, 0° L. 175,00 mm, 30° L. 175,00 mm, 70°

Sinoscope. Otoscope. Sinoscope. L. 175,00 mm, 0° L. 175,00 mm, 30° L. 175,00 mm, 70°. Semiflexible Sinoscope. Flexible Naso-Pharyngoscope. Flexible Naso Pharyngoscope, conventionel bundle, 9800 pixles, 2 ways deflection, one-hand control, soakable, gas-sterilization. Working length 300mm

372 views • 22 slides

m=0;n=0

m=0;n=0. m<1;n=0. m=0;n<1. y(1,1)=-1*1=-1. m=1;n=1. y(2,1)=-2*1+-1*2=-4. m=2;n=1. y(3,1)=-2*2=-4. m=3;n=1. y(1,2)=-1*3+0*1=-3. m=1;n=2. y(2,2)=-2*3+-1*4+3*1+0*2=-7. m=2;n=2. y(3,2)=-2*4+3*2=-2. m=3;n=2. y(1,3)=0*3=0. m=1;n=3. y(2,3)=3*3+0*4=9. m=2;n=3. y(3,3)=3*4=12. m=3;n=3.

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K ±  ±  0  0

K ±  ±  0  0. Measuring Branching Ratio and Dalitz plot parameters. A. Ventura E. Gorini M. Primavera. B ranching Ratio World data.  ’  K ±  ±  0  0. PDG ( units 10 –2 ). 1.73±0.04 (fit) 1.77±0.07 (average). Best experiment is 3.5% error,

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p- K K L m +

p- K K L m +. m + component, ME m + component, LE. Difference. So carried out idea…. Nubar-PID performance on L100200. data and MC. L010185. L100200. data rw-MC. data rw-MC. data (L100200-L010185). rw-MC ( L100200-L010185 ). n (no m +), LE n (no m +), ME.

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k 0

A VCO has a free-running frequency of 120 Mhz and sensitivity (gain) of 20 kHz/v. Assume linear operation over an input range of +/- 5 volts. a. Draw plot of the VCO operating characteristic.

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8 mM ATP 0 mM ATP

Supplemental Fig 2. Wu et al. B. A. P=0.1817 NS (n=3). SI-induced current ( pA/pF ) at -100 mV. SI-induced current ( pA/pF ) at -100 mV. C. P=0.11 NS (n=5). ■ □. 8 mM ATP 0 mM ATP. Supplemental Figure 2. The SI-stimulated channel is pH- and ATP-independent

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ץ + n  K 0 + Λ With decays: K 0  π 0 + π 0 Λ  π 0 + n

Proposal for an Experiment: Photoproduction of Neutral Kaons on Deuterium Spokespersons: D. M. Manley (Kent State University) W. J. Briscoe (The George Washington University) A. Starostin (UCLA). ץ + n  K 0 + Λ With decays: K 0  π 0 + π 0 Λ  π 0 + n

287 views • 18 slides

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Isoaspartyl Peptidase Activity Assay: Determination of Kinetic Parameters with b-Asp-His Substrate

Isoaspartyl Peptidase Activity Assay: Determination of Kinetic Parameters with b-Asp-His Substrate

Presentation Transcript

M A Y 2 0 0 7

M A Y 2 0 0 7

n=4,m=0

Mm, Mm, Mm, Mm is for moon . Mm, Mm, Mm, Mm is for mouse . Mm, / m /, moon .

T M K

K M

D 0  K + K - Signal

Mm, Mm, Mm, Mm is for moon . Mm, Mm, Mm, Mm is for mouse . Mm, / m /, moon .

Treatment Sucrose (mM) Nitrate (mM) 1 30 0 2 30 0 3 60 0 4 60 0 5 90 0 6 90 0

KTeV Results: K L  p 0 ll ( l =e, m , n )

Measurement of the Amplitude Ratio of B 0  J/  K *0 to B 0  J/  K *0 (K +  - ) decays

Search for Super-Penguins: CP Violation in B 0 -&gt;K+K-K 0

Analysis of ratio BR( K     0 )/BR(K    )

D 1 -D 2 lifetime difference using D 0 K  K  , D 0      and D 0 K   

L. 175,00 mm, 0° L. 175,00 mm, 30° L. 175,00 mm, 70°

m=0;n=0

K ±  ±  0  0

p- K K L m +

k 0

8 mM ATP 0 mM ATP

ץ + n  K 0 + Λ With decays: K 0  π 0 + π 0 Λ  π 0 + n

Measurement of the Amplitude Ratio of B 0  J/  K 0 to B 0  J/  K 0 (K +  - ) decays

Search for Super-Penguins: CP Violation in B 0 ->K+K-K 0