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CSHL Computational Cell Biology Islet Assessment. July 2012 Allan J. Ropski University of Illinois at Chicago Department of Surgery. Islets of Langerhans. Islet Potency Index FDA requirement for use as a biological drug Sterility, identity, and purity are established

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cshl computational cell biology islet assessment

CSHL Computational Cell BiologyIslet Assessment

July 2012

Allan J. Ropski

University of Illinois at Chicago

Department of Surgery

islets of langerhans
Islets of Langerhans
  • Islet Potency Index
    • FDA requirement for use as a biological drug
    • Sterility, identity, and purity are established
  • Reliably predict transplant outcomes
    • Glucose-stimulated insulin secretion; β-cell specific
    • In-vivo streptozotocin treated mice; Potency Index
    • UIC-MS islet assays
    • Phase III human clinical trials*; 52-week follow-up
  • Microfluidic optimization
    • Ca2+; Mitochondrial Potential; Insulin release
    • High-throughput assays
human islet cell composition
Human Islet Cell Composition
  • 6 isolations
  • 32 sampled islets
  • Ranges

α-cells 28 – 75%

β-cells 10 – 65%

δ-cells 1 – 22%

Assessment of Human Pancreatic Islet Architecture and Composition by Laser Scanning Confocal Microscopy

Journal of Histochemistry & Cytochemistry; Volume 53(9): 1087–1097, 2005

Marcela Brissova, Michael J. Fowler, Wendell E. Nicholson, Anita Chu, Boaz Hirshberg, David M. Harlan, and Alvin C. Powers