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Unit 7 The History, Structure, Function, and Applications of DNA The History of DNA It took a lot of different scientists a long time to figure out that DNA is the molecule controlling inheritance of genetic traits

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unit 7
Unit 7
  • The History, Structure, Function, and Applications of DNA
the history of dna
The History of DNA
  • It took a lot of different scientists a long time to figure out that DNA is the molecule controlling inheritance of genetic traits
slide3
Soon after chromosomes were discovered, scientists were able to grind them up and learn that they were about 50% protein and 50% nucleic acid - which is DNA.

%

%

DNA

Protein

slide4
Frederick Griffith
    • 1928
    • Experimented with Streptococcus pneumoniae, a bacterium that causes the lungs to fill up with fluid.
      • identified two strains
        • Smooth (S) strain Streptococcus
        • Rough (R) strain Streptococcus
slide5
S strain bacteria appear smooth under the microscope because they have a slimy mucus coating outside their cell walls.
  • This makes them much harder to cough up or for the immune system cells to attack.
slide7
Injected S strain into mice
    • mice died
    • conclusion: S strain is lethal
  • Injected R strain into mice
    • mice survived
    • conclusion: R strain harmless
  • Prediction: The bacteria must have the genetic ability to make mucus to be lethal.
slide8
Injected mice with boiled, heat-killed S strain
    • prediction: mice would survive because the bacteria were dead.
    • observation: mice survived
    • conclusion: Bacteria must be smooth, alive, and reproducing to cause the mice to die.
slide9
Injected mice with a mixture of dead S and living R bacteria
    • prediction: mice would survive
    • observation: mice died
    • conclusion: A new question - what happened?
slide10
Examined blood samples from the mice that died after injection with mixture of dead S and living R bacteria
    • observation: found living S bacteria
    • conclusion: living R are able to absorb a transforming factor from the dead S and transform themselves into S bacteria
    • conclusion: this transformation is passed to new bacteria as they divide, so it must be genetic material.
slide11
Avery and Macleod
    • 1944
    • continued Griffith’s experiment
    • concluded that the transforming factor was probably DNA, but their evidence was not widely accepted by other scientists.
slide12
Hershey and Chase
    • 1952
    • experimented with bacteria and viruses that infect bacteria called bacteriophages
    • knew that bacteriophages are made off only two things, DNA and protein
slide13
Hershey and Chase experiment
    • prediction: bacteriophages must inject their genetic material into their host bacteria cells in order to reproduce.
    • This genetic material must be either the protein or the DNA
slide14
Experimental Group 1.
    • grow bacteriophages and feed them radioactive sulfur
    • this will produces bacteriophages with radioactive protein only, since DNA contains no sulfur
    • Allow the bacteriophages with radioactive protein to infect host bacteria
      • observation: the radioactive protein did not get inside the host bacteria
      • conclusion: the protein is not the genetic material
slide15
Experimental Group 2.
    • grow bacteriophages and feed them radioactive phosphorus
    • this will produces bacteriophages with radioactive DNA only, since protein contains no phosphorus
    • Allow the bacteriophages with radioactive DNA to infect host bacteria
      • observation: the radioactive DNA was injected inside the host bacteria
      • conclusion: the DNA is the genetic material
slide17
Edwin Chargaff
    • 1950’s
    • analyzed the DNA of different animals to figure out if the proportions of adenine, thymine, guanine, and cytosine in their DNA could be used to tell them apart.
slide18
Chargaff’s observations
    • the amount of adenine in any animal’s DNA is always equal to the amount of thymine
    • the amount of guanine in any animal’s DNA is always equal to the amount of cytosine
    • This is called Chargaff’s Rule:
      • amount of A = amount of T
      • amount G = amount of C
slide19
Rosalind Franklin and Maurice Wilkins
    • used x-ray crystallography to photograph DNA crystals
    • produced a diffraction pattern
    • were able to measure the width and distance between repeats in the DNA molecule
    • concluded that the DNA molecule was a certain width with regular repeats
slide20
James Watson and Francis Crick
    • Feb 28,1953
    • figured out the double helix shape of the DNA molecule
slide21
Watson and Crick were the first to put all the pieces together
    • from Chargaff they learned that DNA was made of two purines (adenine and guanine) and two pyrimidines (thymine and cytosine)
    • Chargaff’s rules meant that their was a relationship between A and T, C and G
slide22
From Franklin and Wilkins they learned that DNA was long, skinny, and the same width all the way down
slide24
The Double Helix Model
    • DNA is made of two parallel strands
    • The two strands are held together on the inside by hydrogen bonds between A and T and hydrogen bonds between G and C
    • The two strands twist together in a spiral or helix
    • The outside of each strand is made up of alternating deoxyribose and phosphate groups.
slide27
Beadle and Tatum
    • 1941
    • experimented with mutated molds
    • discovered that a single mutated gene produces a single mutated enzyme
    • mutated enzymes don’t work properly and can cause disease
slide28
The one gene-one protein idea
    • Every different protein or enzyme made in the cell must have its own unique gene stored in the genetic material.
    • defective genes make defective proteins, and this can cause genetic diseases like
      • cystic fibrosis
      • sickle cell anemia
the structure of dna
The Structure of DNA
  • DNA is a double helix molecule
  • Each strand is complementary to the one across from it
  • A only pairs across from T
  • G only pairs across from G
slide30
DNA Replication
    • DNA replication copies DNA
    • occurs during the “S” part of the cell cycle
    • occurs inside the nucleus
    • Each strand acts as a template for the newly forming strand
    • Enzymes work like machines to replicate DNA
slide32
DNA replication- how the enzymes do it
    • 1. DNA helicase unwinds the helix and unzips the two strands by breaking the weak hydrogen bonds
    • 2. DNA polymerases attach to each side and begin adding complementary nucleotides
    • DNA ligases seal the phosphate to sugar bonds
the function of dna
The Function of DNA
  • So far we have covered
    • the history of scientific research proving DNA is the molecule storing hereditary information
    • the structure of DNA and how it copies itself
  • Now we will investigate exactly how DNA codes for proteins.
slide35
DNA controls heredity by coding for how proteins are made
    • enzymes operate cell metabolism
      • examples: catalase, amylase, sucrase, proteases, polymerases, etc.
    • structuralproteins build many cell parts
      • example: keratin builds hair
      • example: actin and myosin build muscle
slide36
DNA is like a set of recipes the cell uses to manufacture just about everything involving proteins
  • Organisms inherit slightly differentrecipes, therefore their proteins are slightly different
  • Protein synthesis is the manufacture of proteins according to recipes.
slide37
Protein Synthesis
    • takes place in the cytoplasm
    • actual work is performed by ribosomes
    • Ribosomes are made of RNA
slide38
RNA
    • made of 4 RNA nucleotides
      • adenine, guanine, cytosine, and uracil
    • single-stranded NOT double stranded
    • contains the sugar ribose instead of deoxyribose.
slide39

DNA

double-stranded

Nitrogen bases = adenine, thymine, guanine, cytosine

stored inside nucleus

built with deoxyribose

RNA

single-stranded

Nitrogen bases = adenine, uracil, guanine, cytosine

made inside nucleus but used in the cytoplasm

built with ribose

slide40
Protein Synthesis takes place in two steps
    • transcription - inside nucleus
    • translation - in cytoplasm
slide41
Transcription
    • A section of DNA containing a gene (protein recipe) unwinds and unzips
    • RNA polymerase builds a strand of RNA complementary to the DNA
slide42
Transcription produces a strand of RNA complementary to the DNA called mRNA
    • mRNA is a temporary “copy” of the gene
    • mRNA can leave the nucleus through pores in the nuclear membrane
    • mRNA can be “read” by the ribosomes in the cytoplasm to make proteins.
slide43
Translation
    • takes place in the cytoplasm
    • a ribosome attaches to the mRNA
    • as the ribosomes slides along, the mRNA strand is read in 3-base long words called codons.
    • Each codon specifies only one amino acid
    • Each codon is matched to a complementary anticodon on a tRNA molecule
    • each tRNA molecule carries the correct amino acid
slide44
Building a polypeptide chain
    • mRNA codons are translated to tRNA anticodons
    • as the tRNA’s line up side by side on the ribosome, they deliver amino acids in sequence
    • Each new amino acid attaches to the one before it with a peptide bond
    • The chain of amino acids is called a polypeptide
slide46
Polypeptides are folded into proteins
    • after completion, most polypeptide chains are moved inside the RER
    • inside the RER, the polypeptide chain is folded into a three-dimensional shape
    • The shape of a protein determines its function
      • enzyme proteins must be folded to produce their active sites
slide47
The codon chart
    • each codon specifies one and only one amino acid
    • Examples
      • UUU codes for phenylalanine (phe)
      • GUG codes for valine (val)
slide49
Some amino acids have up to six different codons, while others have only one
    • Serine(ser) can be specified by the codons UCU, UCA,UCG,UCC, AGU, and AGC
    • Tryptophan has only one codon - UGG
slide50
Signal Codons
    • RNA polymerase needs a signal to know where to start translating the mRNA strand, and a signal to stop translating:
      • AUG is the “start” codon that begins the polypeptide chain with Methionine (met)
      • UAA, AUG, and UGA are “stop” codons that signal the ribosome to let go of the mRNA strand.
mutations
Mutations
  • Mutations occur when the DNA gene’s sequence of base pairs is changed by either a mistake during replication or by a random chemical reaction that damages the DNA
  • Mutations can be harmful, helpful, or silent
slide52
Harmful mutations cause a change in the DNA that produces a defective protein.
  • Helpful mutations are very rare, and cause a change in the DNA that produces a better protein
  • Silent mutations cause a change in the DNA that has no effect on the protein
slide53
A point mutation occurs when a single base pair is removed and a different base pair is substituted.
  • Another name for a point mutation is a substitution
  • Sickle Cell Anemia is caused by a single mutation that changes the codon from GAG to GUG
slide54
A frame-shift mutation occurs when a base pair is deleted or an extra base pair is added.
  • frame-shift mutations are caused by deletions or additions to the gene
  • after a frame-shift mutation, all the codons downstream from the mutation will be read wrong by the ribosomes during translation.
  • frame-shift mutations are always harmful.
slide55
Sentence analogy to show a frame-shift mutation
    • THECAT ATETHERAT = normal
    • _HEC ATA TET HER AT = deletion
    • THE CAT AAT ETH ERA T = addition
slide56
Inversion mutations occur when a section of a gene is cut out and re-inserted into the gene backwards in the same location
slide57
A transposition mutation occurs when a section of a gene is cut out and re-inserted somewhere else.
  • Indian corn kernels are different colors because of a transposition mutation
slide58
A repetitive sequence mutation produces a stretch of DNA that repeats a series of base pairs over and over.
  • Huntingtons Disease is caused by the repetitive sequence mutation “CAG”
    • 10-15 repeats = normal
    • 16-35 repeats = mild symptoms
    • +35 repeats = fatal disease
mutagens
Mutagens
  • Mutagens are environmental factors or chemicals that cause mutations in DNA
  • Mutagens that cause mutations in genes regulating cell division can cause cancer
  • Mutagens that cause cancer are known as carcinogens
slide60
Carcinogens increase the risk of cancer
    • cigarette smoke
    • saccharine
    • asbestos
    • Ultraviolet (UV) radiation
    • X-rays
dna technology
DNA Technology
  • Scientists have used what they know about DNA to change medicine, industry, and agriculture
  • Scientists can now change genetic code
  • Genetic engineering is the manipulation of genetic code to produce combinations of genes
slide62
Recombinant DNA is one kind of genetic engineering
    • DNA from different animals or plants is recombined to produce completely new combinations
    • bacteria, animals or plants are given metabolic abilities they never had before
    • This technology is very controversial
slide63

Scientists can now grow recombinant tobacco plants that glow in the dark because a gene from fireflies was added to their genome.

slide64
Soybeans have been genetically modified so that they are not killed by Roundup, a popular brand of herbicide
slide65
Examples of recombinant DNA
    • bacteria have been engineered to manufacture human insulin for diabetics
slide66
How recombinant DNA techniques were used to create insulin-producing bacteria:
  • 1. locate the insulin gene that you want to move to the bacterial cell
  • 2. cut out the insulin gene using restriction enzymes
  • 3. locate a plasmid from a bacterial cell and cut it with the same restriction enzymes
  • 4. mix the insulin gene and the plasmid DNA
slide67
5. Add DNAligase to the mixture to bond the target gene into the bacterial plasmid DNA
  • 6. Insert the recombinant plasmid into living bacteria.
gel electrophoresis
Gel Electrophoresis
  • Gel electrophoresis is a way to analyze DNA by cutting it up into fragments, then sorting the fragments according to size.
  • Electricity causes the fragments to sort themselves in a pan of special gel
  • Restriction enzymes cut up DNA by breaking it at very specific places only.
slide71
DNA fingerprinting
    • get two samples of DNA that you want to compare
    • cut up each sample using the same restriction enzyme
    • run each sample through gel electropheresis side by side
    • compare the banding patterns
slide72

Suspect 2

Crime Scene Specimen

Suspect 1