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MAMMALIAN RADIATION BIOLOGY COURSE

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MAMMALIAN RADIATION BIOLOGY COURSE. Lecture 1 INTERACTION OF RADIATION WITH BIOLOGICAL SYSTEMS. TOPICS COVERED IN THIS LECTURE. Definition of ionizing radiation Types of ionizing radiation and non-ionizing radiation Definition of LET and quality of ionizing radiation

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slide1
MAMMALIAN RADIATION BIOLOGY COURSE

Lecture 1

INTERACTION OF RADIATION WITH BIOLOGICAL SYSTEMS

slide2
TOPICS COVERED IN THIS LECTURE
  • Definition of ionizing radiation
  • Types of ionizing radiation and non-ionizing radiation
  • Definition of LET and quality of ionizing radiation
  • Generation of free radicals
  • Direct and indirect action of ionizing radiation
slide4
Radiation Biology

A study of the action of ionizing radiation on living things

LD/50 = 4 Gy

4 Gy = 67 calories

67 calories = 3 ml sip of 60°C coffee

30-100 Trillion Cells at Risk

  • Different Cell Types
  • Different Cell Cycle
  • Different Cell Targets
slide5
RADIATION

Radiation is the term given to the energy

transmitted by means of particles or waves.

It can beionizingor non-ionizing

slide6
IONIZING RADIATION

The absorption of energy from radiation in biologic material may lead to excitation or to ionization.

If the radiation has sufficient energy to eject orbital electrons from the atom or molecule, the process is called ionization and that radiation is said to be

Ionizing Radiation

slide7
The important characteristic of ionizing radiation is the localized release of large amounts of energy.

The energy per ionizing event – 33 eV – well enough to break

a chemical bond (ex. C=C bond is 4.9 eV).

slide8
Definition of ionizing radiation
  • Types of ionizing radiation and non-ionizing radiation
  • Definition of LET and quality of ionizing radiation
  • Generation of free radicals
  • Direct and indirect action of ionizing radiation
slide9
IONIZING RADIATION

X-rays and γ-rays do not differ in nature

or in properties, only in the way they are

produced

X-rays (produced extra-nuclearly)

γ-rays (produced intra-nuclearly)

  • Electromagnetic
  • Particular

Electrons

Protons

α-Particles

Neutrons

Deuterons

Heavy charged particles

slide10
X-rays are electromagnetic waves

λ - wavelength

ν - frequency

c - velocity

λν=c

All forms of electromagnetic radiation have the same velocity, but different wavelength, and therefore different frequencies

slide11
Like X-rays, radio waves, radar, radiant heat, laser and visible light are forms of electromagnetic radiation. They have the same velocity but different wavelengths.

The Electromagnetic Spectrum

For example, radio wave have a wavelength of 300m; visible light - 5x10-5; X-rays - 1x10-8cm.

slide12
X-rays may be thought of as electromagnetic waves and-alternatively-

as streams of photons, or “packets” of energy

Each energy packet contains an amount of energy equal

to E=hν, wherehis Planck’s constant and ν is the frequency

If a radiation has a long wavelength, it has a small frequency (λν=c),

and so the energy per photon is small. Conversely, radiation with short

wavelength will have a large frequency and hence the energy per

photon is large.

In their biological effects, electromagnetic radiations are considered

to be ionizing if they have a photon energy in excess of 124 eV, which

corresponds to a wavelength shorter than about 10-6 cm.

slide15
The biologic effect of radiation is determined by the photon size of the energy, not by the amount of energy absorbed. Lethal dose of 4Gy corresponds to only 67 cal of the total energy absorbed

Excess temperature (0C) = 60 - 37 = 23

Volume of coffee consumed to

equal the energy in the LD/50/60 = 67

23

= 3 ml = 1 sip

Mass = 70kg

LD/50/60 = 4Gy

Energy absorbed =

70 x 4 = 280 joules

280 = 67 calories

4.18

Mass = 70 kg

Height lifted to equal the energy in the LD/50/60 = 280

70 x 0.0981

= 0.4 m (16 inches)

slide16
Particulate Radiations

Electrons

Protons

α-Particles

Neutrons

Deuterons

Heavy charged particles

These types of radiation occur in nature and also are used

experimentally, in radiation therapy and diagnostic radiology

alpha radiation
Alpha Radiation ()
  • Nuclei of helium atoms
  • 2 protons and 2 neutrons
  • Heavy, slow, +2 charge
  • Can be accelerated in electrical devices similar to those used for protons
  • High linear energy transfer (LET)
  • Low penetrability
  • They are also emitted during the decay of heavy naturally occuring radionuclides:

210

206

4

Po  Pb + He

84

2

82

slide18
Decay of a Heavy Radionuclide by the Emission of an α-particleThe emission of an α-particle (two protons and two neutrons) decreases the atomic number by two and the mass number by four. Note that the radium has changed to another chemical element, radon, as a consequence of the decay.
beta radiation
Beta Radiation ()
  • Electron emitted from nucleus
  • Light, Fast, -1 charge
  • Can be accelerated to high energies in betatron or linear accelerator. Widely used in cancer therapy
  • Can travel several feet in air and has a medium penetrability
  • The range of beta particle is considerably greater than an alpha particle
  • Beta particle may transfer energy through ionization, excitation and it can produce a Bremsstrahlung radiation (X-rays)
slide20
Alpha particles and beta particles are cosidered directly ionizing because they carry a charge and can, therefore, interact directly with atomic electrons through coulombic forces (i.e. like charges repel each other; opposite charges attract each other).
neutrons n
Neutrons (n)
  • Neutral particle
  • Classified by energy:
    • Thermal neutrons – E < 1eV
    • Fast neutrons – E > 10 keV
    • Indirectly ionizing (no electrical charge). Ionization is caused by charged particles, which are produced during collisions with atomic nuclei
  • Neutrons are also emitted as byproducts of fission of heavy unstable radioactive atoms. With the exception of 209 Bi each nucleus with an atomic number greater than 82 is unstable.
  • Neutrons interaction depends on the neutron energy and the material of the absorber:

Scattering: elastic and inelastic; capture; spallation

slide22
Various trajectories and target

Photon

Neutron

Alpha particle

slide23
Interaction of photons with matter
  • Photons have zero mass, zero charge, and a velocity that is always
  • “c”, the speed of light;
  • They do not steadily lose energy via coulombic interactions with
  • atomic electrons as do charged particles;
  • Photons travel considerable distance before transferring the photon
  • energy to electron energy;
  • Photons are far more penetrating than charged particles of similar
  • energy.
slide24
Absorption of an X-ray photon by the Compton processThe process by which X-ray photons are absorbed depends on the energy of the photons and the chemical composition of the absorber.

At high energies

(100 keV-10 MeV)

characteristic of a

cobalt-60 unit or a

linear accelerator

used for radiotherapy,

the Compton process

dominates

Part of the photon energy is given to the electron as kinetic energy.

The photon proceeds with reduced energy

slide25
Absorption of an X-ray photon by the photoelectric processThe photon gives up its energy entirely; the electron is ejected from the atom. The vacancy is filled either by an electron from an outer orbit or by a free electron from outside the atom. The change in energy is emitted as a photon of characteristic X-rays.
  • It is a predominant mode of
  • photon interaction at:
  • relatively low photon energies
  • high atomic number Z
absorption of neutrons elastic collision
Absorption of neutronselastic collision

Neutrons lose their energy by elastic collision with nuclei of similar mass. In soft tissues interaction of a fast neutron with the hydrogen nuclei (protons) is the dominant process of energy transfer.

Part of the energy of the neutron is given to the proton as kinetic energy. Deflected neutron proceeds with reduced energy.

inelastic scattering
At energies above 6MeV inelastic scattering contributes to energy loss in the absorbing material. The neutron may interact with carbon or oxygen nucleus to produce three of four α-particles. These are known as spallation products which are very important at higher energies.

Inelastic scattering

non ionizing radiation
NON-IONIZING RADIATION
  • Can cause excitation of atoms where electrons jump to higher atomic energy levels but are not removed from the atom:
  • UV light
  • - Lasers
  • Microwave
  • Radio waves
  • - Infrared Waves
slide29
LASER - Light Amplification by Stimulated Emission of Radiation
  • Light - describes all types of energy emitted from a laser; visible and invisible
  • Amplification - of electromagnetic energy necessary to produce a very high intensity laser beam
  • Stimulated Emission - process that occurs at the atomic level, by which electromagnetic energy is amplified
  • Radiation - describes how energy is transferred. Does NOT refer to x-rays or gamma rays.
  • CHARACTERISTICS OF LASER LIGHT
  • Monochromatic - when laser light is passed through a prism, no separation occurs
  • Directional - all laser energy travels in the same direction
  • Coherent - rays of laser energy travel together without interfering with one another
interactions of the laser with matter
Interactions of the Laser with Matter

1. Can be absorbed, reflected or transmitted

2. Reflection of laser energy from material surfaces occurs in 2 ways

- Specular - interacts with smooth surfaces and maintains most of the original beam of energy

- Diffuse - interacts with coarse surfaces and disperses the energy in many directions

3. The further laser energy travels within a given material, the more likely the energy will be absorbed within the material

- The laser wavelength and the material with which it interacts determine what percentage of laser energy will be absorbed, reflected or transmitted

slide32
Definition of ionizing radiation
  • Types of ionizing radiation and non-ionizing radiation
  • Definition of LET and quality of ionizing radiation
  • Generation of free radicals
  • Direct and indirect action of ionizing radiation
slide33
Linear Energy Transfer (LET)

is the energy transferred per unit length of the track.

Unit for LET is keV/µm-kiloelectron volt per micrometer

of unit density material.

L=dE/dl, where dE-energy transferred by a charged particle

of specified energy in traversing a distance of dl

slide34
Optimal Linear Energy Transfer (LET):

Radiation with LET

of 100 keV/µM is the most

efficient in producing

biological damage.

The average separation

between ionizing events

coincides with the diameter

of the DNA double helix

slide35
Sparsely ionizing track of a fast electron in a cloud chamber

Densely ionizing track of an alpha particular in a cloud chamber

The deposition of energy of different types of radiation

  • A 10-MeV proton, typical of the recoil protons produced by high-energy neutrons used for radiotherapy. The track is intermediate in ionization density.
  • A 500-keV proton, produced by lower energy neutrons (e.g., from fission spectrum) or by higher-energy neutrons after multiple collisions. The ionizations form a dense column along the track of the particle.
  • A 1-MeV electron, produced for example, by photons of cobalt-60- -rays. The particle is very sparsely ionizing.
  • A 5-keV electron, typical of secondary electrons produced by x-rays of diagnostic quality. This particle is also sparsely ionizing but a little denser than the higher-energy electron.
the deposition of energy of different types of radiation
The deposition of energy of different types of radiation

dispersion of energy

air

tissue

high LET (, n, ~)

greater radiotoxicity

incident radiation

low LET (, x, ~)

LET = linear energy transfer

slide38
Definition of ionizing radiation
  • Types of ionizing radiation and non-ionizing radiation
  • Definition of LET and quality of ionizing radiation
  • Generation of free radicals
  • Direct and indirect action of ionizing radiation
radiation interaction with water
Radiation interaction with water

The cell is composed of 80% water. The ultimate result of radiation interaction with water molecule is the formation of an ion pair and free radicals. Free radicals have an unpaired electron in their outer shell, a state which confers a high degree of reactivity.

radiation

HOH

HOH+ + e-

HOH + e- HOH-

H+ + OH•*

HOH+

HOH-

OH- + H•

Ion pair (H+, OH-)

HOH

Free Radicals (H•, OH•)

slide40
Free radicals initiate chemical reactions that lead to the production of damage via indirect action in the cell. The following outline summarizes the general sequence of events that occurs in the cell via indirect action:

X-ray photon fast electron (e) ion radical

free radical chemical changes

biologic effect

slide41
Definition of ionizing radiation
  • Types of ionizing radiation and non-ionizing radiation
  • Definition of LET and quality of ionizing radiation
  • Generation of free radicals
  • Direct and indirect action of ionizing radiation
slide42
When ionizing radiation interacts with a cell, ionizations and excitations are produced either in critical biological macromolecules (e.g. DNA) or in the medium in which the cellular organelles are suspended (e.g. water, HOH).

Based on the site of these interactions, the action of radiation on the cell can be classified as either direct or indirect.

slide43
Electromagnetic radiations such as X- and gamma-rays are indirectly ionizing.

In indirect action the critical site is damaged by reactive species produced by ionizations elsewhere in the cell, which in turn damage the target

slide45
Direct action dominates for more densely ionizing radiations such as neutrons because the secondary charged particles produced result in a dense column of ionizations more likely to interact with DNA.
sequence of events in indirect action
Sequence of Events in Indirect Action

T1/2 in sec Incident X-ray photons

10-15 Fast electrons

10-5 Ion radicals

10-5 Free radicals

Macromolecular changes from breakage of chemical bonds

Biological effects

days - cell killing

generation - mutation

years - carcinogenesis

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