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9 mL ED - PowerPoint PPT Presentation


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Dénombrement de la flore du yaourt. Technique de dilution. 0,1 mL . 1 mL . 1 mL . 0,1 mL . 1 mL . 9 mL ED. 9 mL ED. 10 mL ED. 9 mL ED. 10 mL ED. Echantillon = yaourt. d = 10 -1. d = 10 -3. d = 10 -6. d = 10 -7. d = 10 -5. Gamme des dilutions. Ensemencement.

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slide1

Dénombrement de la flore du yaourt

Technique de dilution

0,1 mL

1 mL

1 mL

0,1 mL

1 mL

9 mL ED

9 mL ED

10 mL ED

9 mL ED

10 mL ED

Echantillon = yaourt

d = 10-1

d = 10-3

d = 10-6

d = 10-7

d = 10-5

slide2

Gamme des dilutions

Ensemencement

1 pipette stérile en commençant par la dilution la plus grande

1 mL

1 mL

1 mL

1 mL

1 mL

1 mL

IDEM

Répartir le mL de prélèvement dans le fond de la boîte et recouvrir de gélose MRS en surfusion

slide5

Addition de la gélose en surfusion à 45°C

Solidification des milieux en zone stérile