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9 mL ED

Dénombrement de la flore du yaourt. Technique de dilution. 0,1 mL . 1 mL . 1 mL . 0,1 mL . 1 mL . 9 mL ED. 9 mL ED. 10 mL ED. 9 mL ED. 10 mL ED. Echantillon = yaourt. d = 10 -1. d = 10 -3. d = 10 -6. d = 10 -7. d = 10 -5. Gamme des dilutions. Ensemencement.

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9 mL ED

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  1. Dénombrement de la flore du yaourt Technique de dilution 0,1 mL 1 mL 1 mL 0,1 mL 1 mL 9 mL ED 9 mL ED 10 mL ED 9 mL ED 10 mL ED Echantillon = yaourt d = 10-1 d = 10-3 d = 10-6 d = 10-7 d = 10-5

  2. Gamme des dilutions Ensemencement 1 pipette stérile en commençant par la dilution la plus grande 1 mL 1 mL 1 mL 1 mL 1 mL 1 mL IDEM Répartir le mL de prélèvement dans le fond de la boîte et recouvrir de gélose MRS en surfusion

  3. Prélèvement de l’échantillon

  4. dépôt de l’échantillon au fond de la boîte de Pétri

  5. Addition de la gélose en surfusion à 45°C Solidification des milieux en zone stérile

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