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"Wait! What's in my Mouth?". GCS MINI GRANT 2012-2013 Christal MacLamroc Welborn Academy . DNA Extraction. Procedure for Strawberry DNA 1) Place 1 strawberry in the sandwich bag and push out the air. 2) Using your hands mash the strawberry through the bag.
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"Wait! What's in my Mouth?" GCS MINI GRANT 2012-2013 Christal MacLamroc Welborn Academy
DNA Extraction Procedure for Strawberry DNA 1) Place 1 strawberry in the sandwich bag and push out the air. 2) Using your hands mash the strawberry through the bag. 3) Add two droppers full of DNA extraction solution and continue to mash the strawberry with your hands. 4) Cut a corner in the bag, place the gauze over the cup and press the liquids into your test tube. 5) Add a dropper full of isopropyl alcohol to the test tube. DO NOT MIX THE SOLUTION IN THE TEST TUBE. Procedure for Cheek cell DNA: 1) Use a toothpick and LIGHTLY scratch the inside of your cheeks. 2) Swirl water in your cheek DO NOT DRINK IT and spit the water into a cup. 3) Empty the contents of the cup into a test tube. 4) Add two droppers full of DNA extraction solution into the test tube and mix. 5) Add a dropper full of isopropyl alcohol to the test tube. DO NOT MIX THE SOLUTION IN THE TEST TUBE.
What is DNA??? Looking at Cheek Cells
Does a Strawberry have DNA? Extracting Strawberry Cells Add DNA Extraction Liquid to break open the strawberry cells
Hard at work… After mashing the strawberries they had to be strained. After adding the isopropyl alcohol we wait for the DNA to separate.
YUCK! SCIENCE IS AWESOME!!
We have DNA! DNA
Wait! What’s in my Mouth? Specifics of project including creative aspects: All living things are made up of DNA. DNA, however, is very small and difficult to see. This activity will allow students the ability to explore and visualize DNA in various different living organisms including themselves. In the lab, students will physically extract DNA from strawberries, bananas, kiwis, and their own cheek cells. Students will begin by mixing and creating our lysis solution, which breaks up DNA. The solution is 2L-distilled water, 100-mL detergent and 15g of salt. Using our lysis solution and by adding rubbing alcohol each student will be able to “see” DNA clumps from each fruit and their own cheek cells. Our lab will relate the concept of DNA and living organisms and will provide the groundwork for understanding genetics. What you expect the project to accomplish? I want to provide my students with a distinctive and memorable experience about DNA, which they’ve often heard about but probably never seen. I hope this lab will provide a solid foundation in their Science education and that this inquiry based hands on approach will result in improved performance in the classroom. What are the long-term benefits of the project? Through this lab, experiments will gain a first glance at DNA and it will not be from a picture found in a book. In addition, they will learn the knowledge necessary to conduct basic laboratory techniques. Total amount of money required: $300 Itemization of amount: Palmolive washing detergent 25oz $3.99 Salt 30g $6.99 Fisher brand Kimax Reusable Test Tubes (pack of 72) $210 Thermo Scientific Nalgene Droppers (pack of 100) $59.53 70% Isopropyl Rubbing Alcohol 100ml $10 Total= 290.51 Grade level: 7 Number of students served: 170+ Currently no other funding is available for this project.
