Cytopathic effects (CPE) • Morphological changes occurring in cells due to viral infection is called cytopathic effects. • The virus responsible are called cytopathogenic virus. • CPE depends on various factors • Some viruses cause very little or no CPE in cells of their natural host but can be detected via • Inhibition of replication of another virus • Or presence of viral genome or antigens in cultured cells
Swelling and Clumping Cytomegalovirus infected cells swell and round up Adenovirus infected cells greatly enlarge and clump together in” grape-like” clusters.
Vacuolization Cytoplasmic vacuoles characteristic of polyoma virus
Cell fusion RSV infection induces fusion with adjacent cells to form a syncytia (polykaryocytes)
Inclusion Bodies RSV cytoplasmic inclusions Adenovirus nuclear inclusions Rabies virus cytoplasmic inclusions Negri bodies