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A Comparison of Chilled Stallion Sperm Analysis Methods: Industry Standard vs. SpermVision

This study compares the industry standard method of semen analysis with the use of SpermVision for evaluating measurable parameters of chilled stallion spermatozoa. The Materials and Methods section details the collection of ejaculates, chilling to 5°C, manual assessment of concentration and motility, and SpermVision analysis over 8 fields in 3 chambers using ANOVA and Bonferroni post-hoc tests. The Results and Conclusion sections provide insights into the efficiency and reliability of SpermVision in sperm analysis. References include important works in the field of Theriogenology and Animal Reproduction Science by renowned researchers.

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A Comparison of Chilled Stallion Sperm Analysis Methods: Industry Standard vs. SpermVision

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Presentation Transcript

  1. A comparison between industry standard method of semen analysis and the use of SpermVision for the evaluation of the measurable parameters of chilled stallion spermatozoa Tracey Coop and Amy Mills

  2. Introduction • Equine industry standard

  3. Materials and Method • Ejaculates collected • Chilled to 5oC • Manual assessment • Concentration • Motility • Repeated • SpermVision™ • As above over 8 fields/chamber • 3 chambers • ANOVA • Bonferronipost-hoc

  4. Results

  5. Conclusion

  6. References • Iguer-ouada, M. and Verstegen, J. P. 2001. Theriogenology. 55, 733 – 749. • Klimowicz, M. D., Nizanski, W., Batkowski, F. and Savic, M. A. 2008. Theriogenology. 70, 77 – 82. • Rijsselaere, T., Van Soom, A., Maes, D. and de Kruif, A. 2003. Theriogenology. 60, 1553 – 1568. • Schäfer-Somi, S. and Aurich, C. 2007. Animal Reproduction Science. 102, 1 – 13. • Squires, E. L. 2009. Journal of Equine Veterinary Science. 29, 268 – 273. • Varner, D. D. 2008. Theriogenology. 70, 448 – 462.

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