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QC system Norwegian Microarray Consortium (Inge J)

QC system Norwegian Microarray Consortium (Inge J). Goal: Evaluate quality, allow identification of process step with failure Array design Spike controls – >50 arabidopsis probes – for human, mouse, rat oligo chips (Operon) Negative controls Spike mix Span intensity and ratio spectrum

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QC system Norwegian Microarray Consortium (Inge J)

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  1. QC system Norwegian Microarray Consortium(Inge J) • Goal: • Evaluate quality, allow identification of process step with failure • Array design • Spike controls – >50 arabidopsis probes – for human, mouse, rat oligo chips (Operon) • Negative controls • Spike mix • Span intensity and ratio spectrum • Basis for quality assessment • Transcount (Hovig et al) – statistical analysis aiming to quantify transcript counts • Single spot statistics • MA/IR plot statistic (banana factor etc) • Normalisation based on expression data – statistics based on the spikes? • Normalisation based on spikes – statistics based on expression data? • Quality indicators/reports • Numbers, plots • Incorporate in standard NMC service, internal qc and assurance towards users • Store results in BASE – allow refinement of procedure and identification of trends

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